Galactosylation of chitosan-graft-spermine as a gene carrier for hepatocyte targeting in vitro and in vivo

Cited 21 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ H Kim-
dc.contributor.authorY K Kim-
dc.contributor.authorM T Arash-
dc.contributor.authorS H Hong-
dc.contributor.authorJ H Lee-
dc.contributor.authorB N Kang-
dc.contributor.authorY B Bang-
dc.contributor.authorC S Cho-
dc.contributor.authorDae Yeul Yu-
dc.contributor.authorH L Jiang-
dc.contributor.authorM H Cho-
dc.date.accessioned2017-04-19T09:33:19Z-
dc.date.available2017-04-19T09:33:19Z-
dc.date.issued2012-
dc.identifier.issn1533-4880-
dc.identifier.uri10.1166/jnn.2012.6376ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10899-
dc.description.abstractPolyethyleneimine (PEI) has been described as a highly efcient gene carrier due to its efcient proton sponge effect within endosomes. However, many studies have demonstrated that PEI is toxic and associated with a lack of cell specicity despite high transfection efciency. In order to minimize the toxicity of PEI, we prepared chitosan-graft-spermine (CHI-g-SPE) in a previous study. CHI-g-SPE showed low toxicity and high transfection efciency. However, this compound also had limited target cell specicity. In the present study, we synthesized galactosylated CHI-g-SPE (GCS) because this modied GCS could be delivered specically into the liver due to hepatocyte-specic galactose receptors. The DNA-binding properties of GCS at various copolymer/DNA weight ratios were evaluated by a gel retardation assay. The GCS copolymer exhibited signicant DNA-binding ability and efciently protected DNA from nuclease attack. Using energy-ltered transmission electron microscopy (EF-TEM), we observed dense spherical, nano-sized GCS/DNA complexes with a homogenous distribution. Most importantly, GCS was associated with remarkably low cytotoxicity compared to PEI in HepG2, HeLa, and A549 cells. Moreover, GCS carriers specically delivered the gene-of-interest into hepatocytes in vitro as well as in vivo. Our results suggest that the novel GCS described here is a safe and highly efcient carrier for hepatocyte-targeted gene delivery.-
dc.publisherAmer Scientific Publishers-
dc.titleGalactosylation of chitosan-graft-spermine as a gene carrier for hepatocyte targeting in vitro and in vivo-
dc.title.alternativeGalactosylation of chitosan-graft-spermine as a gene carrier for hepatocyte targeting in vitro and in vivo-
dc.typeArticle-
dc.citation.titleJournal of Nanoscience and Nanotechnology-
dc.citation.number7-
dc.citation.endPage5184-
dc.citation.startPage5178-
dc.citation.volume12-
dc.contributor.affiliatedAuthorDae Yeul Yu-
dc.contributor.alternativeName김지혜-
dc.contributor.alternativeName김유경-
dc.contributor.alternativeNameArash-
dc.contributor.alternativeName홍성호-
dc.contributor.alternativeName이재호-
dc.contributor.alternativeName강빛나-
dc.contributor.alternativeName방용빈-
dc.contributor.alternativeName조청수-
dc.contributor.alternativeName유대열-
dc.contributor.alternativeNameJiang-
dc.contributor.alternativeName조명행-
dc.identifier.bibliographicCitationJournal of Nanoscience and Nanotechnology, vol. 12, no. 7, pp. 5178-5184-
dc.identifier.doi10.1166/jnn.2012.6376-
dc.subject.keywordGalactosylated Chitosan-Graft-Spermine-
dc.subject.keywordGene Therapy-
dc.subject.keywordHepatocyte Targeting-
dc.subject.keywordNon-Viral Gene Delivery-
dc.subject.localGalactosylated Chitosan-Graft-Spermine-
dc.subject.localGene Therapy-
dc.subject.localgene therapy-
dc.subject.localGene therapy-
dc.subject.localHepatocyte targeting-
dc.subject.localHepatocyte Targeting-
dc.subject.localNon-Viral Gene Delivery-
dc.subject.localNon-viral gene delivery-
dc.description.journalClassN-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.