Quantitative proteomic analysis of induced pluripotent stem cells derived from a human Huntington's disease patient

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Title
Quantitative proteomic analysis of induced pluripotent stem cells derived from a human Huntington's disease patient
Author(s)
J I Chae; D W Kim; N Lee; Young Joo Jeon; I Jeon; J Kwon; J Kim; Y Soh; D S Lee; K S Seo; N J Choi; Byoung Chul ParkSunghyun Kang; J Ryu; S H Oh; D A Shin; D R Lee; J T Do; I H Park; G Q Daley; J Song
Bibliographic Citation
Biochemical Journal, vol. 446, no. 3, pp. 359-371
Publication Year
2012
Abstract
HD (Huntington's disease) is a devastating neurodegenerative genetic disorder caused by abnormal expansion of CAG repeats in the HTT (huntingtin) gene.We have recently established two iPSC (induced pluripotent stem cell) lines derived from a HD patient carrying 72 CAG repeats (HD-iPSC). In order to understand the proteomic profiles of HD-iPSCs, we have performed comparative proteomic analysis among normal hESCs (human embryonic stem cells; H9), iPSCs (551-8) and HD-iPSCs at undifferentiated stages, and identified 26 up- and down-regulated proteins. Interestingly, these differentially expressed proteins are known to be involved in different biological processes, such as oxidative stress, programmed cell death and cellular oxygenassociated proteins. Among them, we found that oxidative stressrelated proteins, such as SOD1 (superoxide dismutase 1) and Prx (peroxiredoxin) families are particularly affected in HD-iPSCs, implying that HD-iPSCs are highly susceptible to oxidative stress. We also found that BTF3 (basic transcription factor 3) is upregulated in HD-iPSCs, which leads to the induction of ATM (ataxia telangiectasia mutated), followed by activation of the p53- mediated apoptotic pathway. In addition, we observed that the expression of cytoskeleton-associated proteins was significantly reduced in HD-iPSCs, implying that neuronal differentiation was also affected. Taken together, these results demonstrate that HDiPSCs can provide a unique cellular disease model system to understand the pathogenesis and neurodegeneration mechanisms in HD, and the identified proteins from the present study may serve as potential targets for developing future HD therapeutics.
Keyword
ApoptosisCytoskeleton-associated proteinsHuntington's diseaseInduced pluripotent stem cell (iPSC)Oxidative stressProteomic analysis
ISSN
0264-6021
Publisher
Portland Press Ltd
DOI
http://dx.doi.org/10.1042/BJ20111495
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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