Marinomonas hwangdonensis sp. nov., isolated from seawater

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, designated HDW-15T, was isolated from seawater of the Yellow Sea, Korea, and subjected to a polyphasic taxonomic study. Strain HDW-15 T grew optimally at pH 7.0-8.0, at 25°C and in the presence of 2% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HDW-15 T fell within the clade comprising Marinomonas species, joining the type strain of Marinomonas arctica, with which it exhibited highest 16S rRNA gene sequence similarity (97.7 %). The 16S rRNA gene sequence similarity values between strain HDW-15 T and the type strains of other Marinomonas species were in the range 93.7-97.2 %. Mean DNA-DNA relatedness values between strain HDW-15 T and the type strains of M. arctica, Marinomonas polaris and Marinomonas pontica were 5.0-9.9 %. The DNA G+C content of the isolate was 48.7 mol%. Strain HDW-15 T contained Q-8 as the predominant ubiquinone and C 18: 1ω7c, summed feature 3 (C 16: 1 ω 7c and/ or iso-C 15: 0 2-OH) and C 16: 0 as the major fatty acids. The major polar lipids found in strain HDW-15 T were phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, showed that strain HDW-15 T can be differentiated from other Marinomonas species. On the basis of the data presented, strain HDW-15 T is considered to represent a novel species of the genus Marinomonas, for which the name Marinomonas hwangdonensis sp. nov. is proposed. The type strain is HDW-15 T (=KCTC 23661 T=CCUG 61321 T).