Virulence and transmissibility of H1N2 influenza virus in ferrets imply the continuing threat of triple-reassortant swine viruses

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dc.contributor.authorP N Q Pascuaa-
dc.contributor.authorM S Song-
dc.contributor.authorJ H Lee-
dc.contributor.authorY H Baek-
dc.contributor.authorH I Kwon-
dc.contributor.authorS J Park-
dc.contributor.authorE H Choi-
dc.contributor.authorG J Lim-
dc.contributor.authorO J Lee-
dc.contributor.authorS W Kim-
dc.contributor.authorC J Kim-
dc.contributor.authorM H Sung-
dc.contributor.authorMyung Hee Kim-
dc.contributor.authorSun Woo Yoon-
dc.contributor.authorE A Govorkova-
dc.contributor.authorR J Webby-
dc.contributor.authorR G Webster-
dc.contributor.authorY K Choi-
dc.date.accessioned2017-04-19T09:33:53Z-
dc.date.available2017-04-19T09:33:53Z-
dc.date.issued2012-
dc.identifier.issn0027-8424-
dc.identifier.uri10.1073/pnas.1205576109ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10945-
dc.description.abstractEfficient worldwide swine surveillance for influenza A viruses is urgently needed; the emergence of a novel reassortant pandemic H1N1 (pH1N1) virus in 2009 demonstrated that swine can be the direct source of pandemic influenza and that the pandemic potential of viruses prevalent in swine populations must be monitored. We used the ferret model to assess the pathogenicity and transmissibility of predominant Korean triple-reassortant swine (TRSw) H1N2 and H3N2 influenza viruses genetically related to North American strains. Although most of the TRSw viruses were moderately pathogenic, one [A/Swine/Korea/1204/ 2009; Sw/1204 (H1N2)] was virulent in ferrets, causing death within 10 d of inoculation, and was efficiently transmitted to naive contact ferrets via respiratory droplets. Although molecular analysis did not reveal known virulence markers, the Sw/1204 virus acquired mutations in hemagglutinin (HA) (Asp-225-Gly) and neuraminidase (NA) (Ser-315-Asn) proteins during the single ferret passage. The contact-Sw/1204 virus became more virulent in mice, replicated efficiently in vitro, extensively infected human lung tissues ex vivo, and maintained its ability to replicate and transmit in swine. Reverse-genetics studies further indicated that the HA 225G and NA 315N substitutions contributed substantially in altering virulence and transmissibility. These findings support the continuing threat of some field TRSw viruses to human and animal health, reviving concerns on the capacity of pigs to create future pandemic viruses. Apart from warranting continued and enhanced global surveillance, this study also provides evidence on the emerging roles of HA 225G and NA 315N as potential virulence markers in mammals.-
dc.publisherNatl Acad Sciences-
dc.titleVirulence and transmissibility of H1N2 influenza virus in ferrets imply the continuing threat of triple-reassortant swine viruses-
dc.title.alternativeVirulence and transmissibility of H1N2 influenza virus in ferrets imply the continuing threat of triple-reassortant swine viruses-
dc.typeArticle-
dc.citation.titleProceedings of National Academy of Sciences of United States of America-
dc.citation.number39-
dc.citation.endPage15905-
dc.citation.startPage15900-
dc.citation.volume109-
dc.contributor.affiliatedAuthorMyung Hee Kim-
dc.contributor.affiliatedAuthorSun Woo Yoon-
dc.contributor.alternativeNamePascuaa-
dc.contributor.alternativeName송민석-
dc.contributor.alternativeName이준한-
dc.contributor.alternativeName백윤희-
dc.contributor.alternativeName권혁일-
dc.contributor.alternativeName박수진-
dc.contributor.alternativeName최은혜-
dc.contributor.alternativeName임교진-
dc.contributor.alternativeName이옥준-
dc.contributor.alternativeName김시욱-
dc.contributor.alternativeName김철중-
dc.contributor.alternativeName성문희-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName윤선우-
dc.contributor.alternativeNameGovorkova-
dc.contributor.alternativeNameWebby-
dc.contributor.alternativeNameWebster-
dc.contributor.alternativeName최영기-
dc.identifier.bibliographicCitationProceedings of National Academy of Sciences of United States of America, vol. 109, no. 39, pp. 15900-15905-
dc.identifier.doi10.1073/pnas.1205576109-
dc.subject.keywordEvolution-
dc.subject.keywordInterspecies transmission-
dc.subject.keywordSevere disease-
dc.subject.keywordViral segments-
dc.subject.localEvolution-
dc.subject.localevolution-
dc.subject.localInterspecies transmission-
dc.subject.localinterspecies transmission-
dc.subject.localinterspecies trasnmission-
dc.subject.localSevere disease-
dc.subject.localSevere Disease-
dc.subject.localViral segments-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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