Analysis of carotenoid accumulation and expression of carotenoid biosynthesis genes in different organs of Chinese cabbage (Brassica rapa subsp. pekinensis)

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dc.contributor.authorP A Tuan-
dc.contributor.authorJ K Kim-
dc.contributor.authorJeongyeo Lee-
dc.contributor.authorW T Park-
dc.contributor.authorD Y Kwon-
dc.contributor.authorY B Kim-
dc.contributor.authorH H Kim-
dc.contributor.authorHyeran Kim-
dc.contributor.authorS U Park-
dc.date.accessioned2017-04-19T09:34:23Z-
dc.date.available2017-04-19T09:34:23Z-
dc.date.issued2012-
dc.identifier.issn16112156-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10975-
dc.description.abstractThe relationship between carotenoid accumulation and expression of carotenoid biosynthesis genes was investigated in the flowers, stems, young leaves, old leaves, and roots of Chinese cabbage (Brassica rapa subsp. pekinensis). Quantitative real-time PCR analysis showed that the mRNA levels of BrPSY, BrPDS, BrZDS, BrLCYB, BrLCYE, BrCHXB, and BrZEP leading to the production of carotenoids were highest in the flowers or the leaves and lowest in the roots of Chinese cabbage. In contrast, the mRNA expression of BrNCED, a gene involved in abscisic acid (ABA) biosynthesis, was highest in the roots. High-performance liquid chromatography revealed that carotenoids, namely, lutein and β-carotene, were distributed predominantly in the flowers and leaves, with very little in the underground organ, the roots. Specifically, old leaves contained 120.3 μg/g lutein and 103.93 μg/g β-carotene, which is the most potent dietary precursor of vitamin A. Moreover, we found a relatively large amount of cis isomers of β-carotene, namely, 9-cis β-carotene and 13-cis β-carotene, in Chinese cabbage. These results provide insight into carotenoid biosynthetic mechanisms in Chinese cabbage and may be helpful in the metabolic engineering of carotenoid biosynthesis in plants.-
dc.publisherExcli Journal Managing Office-
dc.titleAnalysis of carotenoid accumulation and expression of carotenoid biosynthesis genes in different organs of Chinese cabbage (Brassica rapa subsp. pekinensis)-
dc.title.alternativeAnalysis of carotenoid accumulation and expression of carotenoid biosynthesis genes in different organs of Chinese cabbage (Brassica rapa subsp. pekinensis)-
dc.typeArticle-
dc.citation.titleExcli Journal-
dc.citation.number0-
dc.citation.endPage516-
dc.citation.startPage508-
dc.citation.volume11-
dc.contributor.affiliatedAuthorJeongyeo Lee-
dc.contributor.affiliatedAuthorHyeran Kim-
dc.contributor.alternativeNameTuan-
dc.contributor.alternativeName김재광-
dc.contributor.alternativeName이정여-
dc.contributor.alternativeName박우태-
dc.contributor.alternativeName권도연-
dc.contributor.alternativeName김연복-
dc.contributor.alternativeName김행훈-
dc.contributor.alternativeName김혜란-
dc.contributor.alternativeName박상운-
dc.identifier.bibliographicCitationExcli Journal, vol. 11, pp. 508-516-
dc.subject.keywordβ-carotene-
dc.subject.keywordBrassica rapa subsp. pekinensis-
dc.subject.keywordCarotenoid biosynthesis genes-
dc.subject.keywordCarotenoids-
dc.subject.keywordChinese cabbage-
dc.subject.localβ-carotene-
dc.subject.localBrassica rapa subsp. pekinensis-
dc.subject.localCarotenoid biosynthesis genes-
dc.subject.localCarotenoids-
dc.subject.localCarotenoid-
dc.subject.localChinese cabbage-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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