Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4

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Title
Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4
Author(s)
H S Kim; Y J Kim; H K Lee; H S Ryu; J S Kim; M J Yoon; Jong Soon Kang; J T Hong; Y Kim; S B Han
Bibliographic Citation
Food and Chemical Toxicology, vol. 50, no. 9, pp. 3190-3197
Publication Year
2012
Abstract
Paecilomyces cicadae have been reported to have immunomodulatory properties. In this study, we investigated the effect of polysaccharide (PCP) isolated from P. cicadae on the macrophages. PCP increased the production of nitric oxide (NO) and the gene expression of IL-1β, IL-6, and TNF-α in RAW 264.7 cells. To investigate the membrane receptor, we examined the effect of PCP on primary macrophages isolated from wild type C3H/HeN and C3H/HeJ mice having mutant-TLR4. PCP induced NO production and cytokine gene expression in macrophages from C3H/HeN, but not from tlr4-mutated C3H/HeJ mice, which suggests that TLR4 is the membrane receptor for PCP. PCP induced the phosphorylation of ERK, JNK, and p38, and the nuclear translocation of NF-κB p50/p65, which are the main signaling molecules downstream from TLR4. Among them, p38 and NF-κB signaling played a crucial role in PCP-induced NO production by macrophages. These results indicate that PCP activates macrophages through the TLR4 signaling pathway.
Keyword
MacrophageMAPKNF-κBPaecilomyces cicadaePolysaccharideTLR4
ISSN
0278-6915
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.fct.2012.05.051
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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