A nobel gebberellin 2-oxidase gene CaGA2ox1 in pepper is specifically induced by incompatible plant pathogens = 고추 GA2-oxidase 유전자 연구

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Title
A nobel gebberellin 2-oxidase gene CaGA2ox1 in pepper is specifically induced by incompatible plant pathogens = 고추 GA2-oxidase 유전자 연구
Author(s)
Yeon Lee; Young-Cheol Kim; Soo Yong Kim; I J Lee; D Choi; K H Paek; Hye Sun ChoSuk Yoon KwonJeong Mee Park
Bibliographic Citation
Plant Biotechnology Reports, vol. 6, no. 4, pp. 381-390
Publication Year
2012
Abstract
Phytohormone balance is increasingly recognized as central to the outcome of plant-pathogen interactions. Differential screening for genes induced by a non-host pathogen in pepper plants (Capsicum annuum) identified a putative gibberellin 2-oxidase gene, CaGA2ox1. Analysis of the deduced amino acid sequence of CaGA2ox1 showed 53 and 50 % amino acid identity to Pisum sativum PsGA2ox2 and Arabidopsis AtGA2ox6, respectively. Expression in pepper plants of CaGA2ox1 was preferentially increased in response to non-host pathogeninoculation and during the host resistance response. CaGA2ox1 expression increased following treatment with salicylic acid and ethephon (albeit with different induction patterns), but remained unchanged following treatment with methyl jasmonate and abscisic acid. The gene product of CaGA2ox1 is predicted to catalyze the metabolism of GA 4, and does so in recombinant E. coli extracts. Further PEG-mediated transient expression studies showed that CaGA2ox1 fused with soluble modified green fluorescent protein localized to the cytosol in chili pepper protoplasts. Interestingly, the transcript level of CaGA2ox1 was not affected by treatments of either pepper with bioactive GA 4+7 or paclobutrazol, an inhibitor of GA biosynthesis. Taken together, these results provide the first evidence that a GA 2-oxidase, which is important in GA metabolism, may also play a role in plant defense signaling and plant-microbe interactions.
Keyword
CaGA2ox1Chili pepper (Capsicum annum)Gibberellin (GA)Non-host pathogen
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-012-0235-2
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > International Biological Material Research Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
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