Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition = EGCG와 그 배당체의 인간 장내 말테이즈의 저해효과

Cited 17 time in scopus
Metadata Downloads
Title
Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition = EGCG와 그 배당체의 인간 장내 말테이즈의 저해효과
Author(s)
T T H Nguyen; S H Jung; S Lee; H J Ryu; H K Kang; Y H Moon; Young Min Kim; A Kimur; D Kim
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 17, no. 5, pp. 966-971
Publication Year
2012
Abstract
Human intestinal maltase (HMA) is an α- glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 μM, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (Ki) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 μM, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.
Keyword
Alpha-glucosidaseEGCGEGCG glucosideHuman intestinal maltaseMolecular docking
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12257-012-0242-8
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.