Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition = EGCG와 그 배당체의 인간 장내 말테이즈의 저해효과
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- Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition = EGCG와 그 배당체의 인간 장내 말테이즈의 저해효과
- T T H Nguyen; S H Jung; S Lee; H J Ryu; H K Kang; Y H Moon; Young Min Kim; A Kimur; D Kim
- Bibliographic Citation
- Biotechnology and Bioprocess Engineering, vol. 17, no. 5, pp. 966-971
- Publication Year
- Human intestinal maltase (HMA) is an α- glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 μM, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (Ki) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 μM, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.
- Alpha-glucosidaseEGCGEGCG glucosideHuman intestinal maltaseMolecular docking
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