Stable expression of a fungal laccase protein using transplastomic tobacco

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dc.contributor.authorS J Davarpana-
dc.contributor.authorJoon Woo Ahn-
dc.contributor.authorS M Ko-
dc.contributor.authorS H Jung-
dc.contributor.authorY I Park-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorWon Joong Jeong-
dc.date.accessioned2017-04-19T09:34:30Z-
dc.date.available2017-04-19T09:34:30Z-
dc.date.issued2012-
dc.identifier.issn1863-5466-
dc.identifier.uri10.1007/s11816-012-0225-4ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10996-
dc.description.abstractLaccase catalyzes the oxidation of various phenolic compounds that can be used in a wide range of industrial applications such as waste detoxification and the textile industry. In the present study, we generated transplastomic tobacco plants to develop a reliable commercial source of laccase production. The stability of the laccase protein in the transgenic plants was increased by using the enhancer sequence from green fluorescent protein, resulting in three independent lines with high levels of laccase accumulation (up to 2 % of total protein); significant laccase activity, however, was not detected. Interestingly, the transplastomic lines showed slightly retarded vegetative growth, with a light green leaf color in comparison with the control, which may be attributable to copper deficiency induced by ligand chelation by abundantly produced laccase. These results suggest that the tobacco chloroplast is an efficient system for the mass production of laccase protein, but further studies are needed to obtain active enzyme.-
dc.publisherSpringer-
dc.titleStable expression of a fungal laccase protein using transplastomic tobacco-
dc.title.alternativeStable expression of a fungal laccase protein using transplastomic tobacco-
dc.typeArticle-
dc.citation.titlePlant Biotechnology Reports-
dc.citation.number4-
dc.citation.endPage312-
dc.citation.startPage305-
dc.citation.volume6-
dc.contributor.affiliatedAuthorJoon Woo Ahn-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.affiliatedAuthorWon Joong Jeong-
dc.contributor.alternativeNameDavarpana-
dc.contributor.alternativeName안준우-
dc.contributor.alternativeName고석민-
dc.contributor.alternativeName정서희-
dc.contributor.alternativeName박연일-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName정원중-
dc.identifier.bibliographicCitationPlant Biotechnology Reports, vol. 6, no. 4, pp. 305-312-
dc.identifier.doi10.1007/s11816-012-0225-4-
dc.subject.keywordHeterologous expression-
dc.subject.keywordHomoplasmy-
dc.subject.keywordLaccase-
dc.subject.keywordTransplastomic plant-
dc.subject.localheterologous expression-
dc.subject.localHeterologous expression-
dc.subject.localHomoplasmy-
dc.subject.locallaccase-
dc.subject.localLaccase-
dc.subject.localTransplastomic plant-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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