Transient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana = 오이 모자이크 바이러스 2b 억제에 의한 EPO의 임시발현

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dc.contributor.authorKi Beom Moon-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorW S Lee-
dc.contributor.authorHyun Soon Kim-
dc.date.accessioned2017-04-19T09:34:53Z-
dc.date.available2017-04-19T09:34:53Z-
dc.date.issued2012-
dc.identifier.citationHorticulture, Environment, and Biotechnology ,53,5,434,440ko
dc.identifier.issn02536498-
dc.identifier.uri10.1007/s13580-012-0971-9ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11008-
dc.description.abstractErythropoietin (EPO) is a glycoprotein hormone that regulates red blood cell formation in mammals. It also plays an important role in wound healing and the brain's response to neuronal injury. Recombinant EPO has been produced in various hosts including Escherichia coli, insect cells, and yeast. Plant systems are also useful for the production of recombinant proteins. In this study, we used Nicotiana benthamiana for the production of recombinant human EPO (rhEPO) using an Agrobacterium-mediated transient expression system. We evaluated the effect of repeated agroinfiltration and Agrobacterium density on the rhEPO production. In addition, the rhEPO expression vector was coinfiltrated with a vector expressing cucumber mosaic virus suppressor 2b (CMV2b), which suppresses posttranscriptional gene silencing. We found that rhEPO expression was increased approximately 5. 5-fold in N. benthamiana leaves coinfiltrated with CMV2b. In contrast, neither Agrobacterium density nor the number of infiltrations influenced rhEPO expression.-
dc.publisherSouth Korea-
dc.titleTransient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana = 오이 모자이크 바이러스 2b 억제에 의한 EPO의 임시발현-
dc.title.alternativeTransient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana-
dc.typeArticle-
dc.citation.titleHorticulture Environment and Biotechnology-
dc.citation.number5-
dc.citation.endPage440-
dc.citation.startPage434-
dc.citation.volume53-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.affiliatedAuthorHyun Soon Kim-
dc.contributor.alternativeName문기범-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName이우성-
dc.contributor.alternativeName김현순-
dc.identifier.bibliographicCitationHorticulture Environment and Biotechnology, vol. 53, no. 5, pp. 434-440-
dc.identifier.doi10.1007/s13580-012-0971-9-
dc.subject.keywordAgrobacterium-
dc.subject.keywordbiopharmaceuticals-
dc.subject.keywordposttranscriptional gene silencing-
dc.subject.keywordrecombinant proteins-
dc.subject.localAgrobacterium-
dc.subject.localbiopharmaceuticals-
dc.subject.localBiopharmaceutical-
dc.subject.localposttranscriptional gene silencing-
dc.subject.localrecombinant proteins-
dc.subject.localrecombinant protein-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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