Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

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Title
Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
Author(s)
M Cha; S H Shim; S H Kim; O T Kim; S W Lee; Suk Yoon Kwon; K H Baek
Bibliographic Citation
BMB Reports, vol. 45, no. 10, pp. 589-594
Publication Year
2012
Abstract
Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 μg taxadiene per gram of dry weight. In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 μg per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene.
Keyword
Panax ginseng C.A. MeyerRoot cultureTaxadiene synthaseTaxolTransformation
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/BMBRep.2012.45.10.085
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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