Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability

Cited 13 time in scopus
Metadata Downloads
Title
Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability
Author(s)
S J Lee; Sang Jun Lee; Yong-Jik Lee; S B Kim; S K Kim; D W Lee
Bibliographic Citation
Applied and Environmental Microbiology, vol. 78, no. 24, pp. 8813-8816
Publication Year
2012
Abstract
To study the pH dependence of L-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pHopt) for activity, whereas the N-terminal surface R residues play an important role in determining the pHopt for stability.
ISSN
0099-2240
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/AEM.02114-12
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.