Genome-wide mRNA profiling and multiplex quantitative RT-PCR for forensic body fluid identification

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dc.contributor.authorSeong-Min Park-
dc.contributor.authorS Y Park-
dc.contributor.authorJeong Hwan Kim-
dc.contributor.authorT W Kang-
dc.contributor.authorJong Lyul Park-
dc.contributor.authorK M Woo-
dc.contributor.authorJ S Kim-
dc.contributor.authorH C Lee-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorS H Lee-
dc.date.accessioned2017-04-19T09:35:48Z-
dc.date.available2017-04-19T09:35:48Z-
dc.date.issued2013-
dc.identifier.issn1872-4973-
dc.identifier.uri10.1016/j.fsigen.2012.09.001ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11073-
dc.description.abstractIn forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges. Messenger RNA (mRNA) profile clearly reveals tissue-specific gene expression patterns that many attempts have been made to use RNA for forensic tissue identification. To systematically investigate the body-fluid-specific expression of mRNAs and find novel mRNA markers for forensic body fluid identification, we performed DNA microarray experiment with 24 Korean body fluid samples. Shannon entropy and Q-values were calculated for each gene, and 137 body-fluid-specific candidate genes were selected. By applying more stringent criteria, we further selected 28 candidate genes and validated them by RT-PCR and qRT-PCR. As a result, we suggest a novel combination of four body-fluid-specific mRNA makers: PPBP for blood, FDCSP for saliva, MSMB for semen and MSLN for vaginal secretion. Multiplex qRT-PCR assay was designed using the four mRNA markers and DNA/RNA co-extraction method was tested for forensic use. This study will provide a thorough examination of body-fluid-specifically expressed mRNAs, which will enlarge the possibility of practical use of RNA for forensic purpose.-
dc.publisherElsevier-
dc.titleGenome-wide mRNA profiling and multiplex quantitative RT-PCR for forensic body fluid identification-
dc.title.alternativeGenome-wide mRNA profiling and multiplex quantitative RT-PCR for forensic body fluid identification-
dc.typeArticle-
dc.citation.titleForensic Science International-Genetics-
dc.citation.number1-
dc.citation.endPage150-
dc.citation.startPage143-
dc.citation.volume7-
dc.contributor.affiliatedAuthorSeong-Min Park-
dc.contributor.affiliatedAuthorJeong Hwan Kim-
dc.contributor.affiliatedAuthorJong Lyul Park-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.alternativeName박성민-
dc.contributor.alternativeName박성연-
dc.contributor.alternativeName김정환-
dc.contributor.alternativeName강태욱-
dc.contributor.alternativeName박종열-
dc.contributor.alternativeName우광만-
dc.contributor.alternativeName김종식-
dc.contributor.alternativeName이한철-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName이승환-
dc.identifier.bibliographicCitationForensic Science International-Genetics, vol. 7, no. 1, pp. 143-150-
dc.identifier.doi10.1016/j.fsigen.2012.09.001-
dc.subject.keywordBody fluid identification-
dc.subject.keywordDNA/RNA co-extraction-
dc.subject.keywordMicroarray-
dc.subject.keywordmRNA marker-
dc.subject.keywordqRT-PCR-
dc.subject.localBody fluid identification-
dc.subject.localDNA/RNA co-extraction-
dc.subject.localmicroarray-
dc.subject.localmicroarry-
dc.subject.localMicroarray-
dc.subject.localmicroarrays-
dc.subject.localmRNA marker-
dc.subject.localqRT-PCR-
dc.subject.localQ-RT-PCR-
dc.description.journalClassY-
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