Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells = 미니돼지의 대동맥 내피세포를 이용한 갱글리오 사이드의 발현 분석

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ H Cho-
dc.contributor.authorJi Su Kim-
dc.contributor.authorM U Lim-
dc.contributor.authorH K Min-
dc.contributor.authorD H Kwak-
dc.contributor.authorJ S Ryu-
dc.contributor.authorJ T Lee-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorC H Kim-
dc.contributor.authorC H Kim-
dc.contributor.authorD B Koo-
dc.contributor.authorKyu Tae Chang-
dc.contributor.authorY K Choo-
dc.date.accessioned2017-04-19T09:36:12Z-
dc.date.available2017-04-19T09:36:12Z-
dc.date.issued2012-
dc.identifier.issn1738-6055-
dc.identifier.uri10.5625/lar.2012.28.4.255ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11134-
dc.description.abstractGangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs, we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-α. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased in PAECs incubated for 5 h with TNF-α (10 ng/mL), 10% human serum containing human leukocytes, and 10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of PAECs with TNF-α. This finding may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.-
dc.publisherSpringer-BMC-
dc.titleHuman leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells = 미니돼지의 대동맥 내피세포를 이용한 갱글리오 사이드의 발현 분석-
dc.title.alternativeHuman leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells-
dc.typeArticle-
dc.citation.titleLaboratory Animal Research-
dc.citation.number4-
dc.citation.endPage263-
dc.citation.startPage255-
dc.citation.volume28-
dc.contributor.affiliatedAuthorJi Su Kim-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.alternativeName조진형-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName임맑음-
dc.contributor.alternativeName민현기-
dc.contributor.alternativeName곽동훈-
dc.contributor.alternativeName류재성-
dc.contributor.alternativeName이주택-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName김장환-
dc.contributor.alternativeName김창현-
dc.contributor.alternativeName구덕본-
dc.contributor.alternativeName장규태-
dc.contributor.alternativeName추영국-
dc.identifier.bibliographicCitationLaboratory Animal Research, vol. 28, no. 4, pp. 255-263-
dc.identifier.doi10.5625/lar.2012.28.4.255-
dc.subject.keywordMicro-pig aortic endothelial cells-
dc.subject.keywordhuman serum-
dc.subject.keywordhuman leukocyte-
dc.subject.keywordtumor necrosis factor-α-
dc.subject.keywordganglioside GM1-
dc.subject.localMicro-pig aortic endothelial cells-
dc.subject.localHuman serum-
dc.subject.localhuman serum-
dc.subject.localhuman leukocyte-
dc.subject.localTumor necrosis fa tor-α-
dc.subject.localTNFα-
dc.subject.localTumor necrosis factor (TNF)-α-
dc.subject.localTnf-α-
dc.subject.localTNF-a-
dc.subject.localTNF-alpha-
dc.subject.localTumor necrosis factor-α-
dc.subject.localtumor necrosis factor-alpha-
dc.subject.localTNFa-
dc.subject.localTumor necrosis factor alpha-
dc.subject.localTumor necrosis factor-alpha-
dc.subject.localTNF-α-
dc.subject.localtumor necrosis factor-α-
dc.subject.localganglioside GM1-
dc.subject.localGanglioside GM1-
dc.description.journalClassN-
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.