Altered ganglioside patterns accompany the anti-inflammatory activity of luteolin in lipopolysaccharide-stimulated Raw 264.7 = 리포폴리 사카아리드가 처리된 RAW264.7에서 루테올린에 의한 갱글리오시드의 발현변화와 항염증 반응

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Title
Altered ganglioside patterns accompany the anti-inflammatory activity of luteolin in lipopolysaccharide-stimulated Raw 264.7 = 리포폴리 사카아리드가 처리된 RAW264.7에서 루테올린에 의한 갱글리오시드의 발현변화와 항염증 반응
Author(s)
J S Ryu; M U Lim; M R Hwang; H K Min; G Moussavou; Ji Su KimSun-Uk Kim; K Ko; Y H Cho; S Y Nam; Kyu Tae Chang; Y K Choo
Bibliographic Citation
Journal of Biomedical Research, vol. 13, no. 4, pp. 287-294
Publication Year
2012
Abstract
Flavonoids have a range of biological activities, including anti-allergic, anti-inflammatory, anti-microbial, and anti-cancer activities, as shown by in vitro studies. In this study, we investigated whether luteolin can be applied to the suppression of lipopolysaccharide (LPS)-stimulated inflammatory responses in murine macrophages. Luteolin was found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells. moreover, expression of inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2) and pro-inflammatory cytokine tumor necrotic factor-α (TNF-α) at the mRNA and protein levels were decreased. These inhibitory effects were found to be caused by the blockage of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation and the phosphorylation of mitogen-activated protein (MAP) kinase famaily, extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK) and p38 MAP kinase. Furthermore, pre-treatment with luteolin reduced ganglioside expression levels and inhibited GT1b expression in Raw 264.7 cells. On the basis of these observations, we suggest that luteolin has potential as an anti-inflammatory drug candidate, and ganglioside GT1b may play a role in the inflammatory.
Keyword
luteolininflammationlipopolysaccharidegangliosidesRaw 264.7 macrophage
ISSN
1976-7447
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.12729/jbr.2012.13.4.287
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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