Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris

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Title
Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris
Author(s)
Jimin Lee; Dae-Hyun Cho; R Ramanan; Byung-Hyuk Kim; Hee-Mock OhHee-Sik Kim
Bibliographic Citation
Bioresource Technology, vol. 131, pp. 195-201
Publication Year
2013
Abstract
Flocculation is most preferred method for harvesting microalgae, however, the role of bacteria in microalgal flocculation process is still unknown. This study proves that bacteria play a profound role in flocculating by increasing the floc size resulting in sedimentation of microalgae. A flocculating activity of 94% was achieved with xenic Chlorella vulgaris culture as compared to 2% achieved with axenic culture. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene of xenic C. vulgaris culture revealed the presence of Flavobacterium sp., Terrimonas sp., Sphingobacterium sp., Rhizobium sp. and Hyphomonas sp. as microalgae-associated bacteria. However when Flavobacterium, Terrimonas, Sphingobacterium were eliminated by fluorescence activated cell sorter (FACS), flocculating activity reduced to 3%. Further studies with cell free extracts also suggest that bacterial extracellular substances might also have a role in enhancing flocculation. We conclude that the collective presence of certain bacteria is the determining factor in flocculation of C. vulgaris.
Keyword
AlgaeChlorella vulgarisFlocculationMicroalgae-associated bacteria
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2012.11.130
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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