GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae Δ strain at anaerobic alcoholic fermentation

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Title
GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae Δ strain at anaerobic alcoholic fermentation
Author(s)
Jungoh Ahn; Kyung Min Park; Hong-Weon Lee; Y J Son; Eui Sung Choi
Bibliographic Citation
FEMS Yeast Research, vol. 13, no. 1, pp. 140-142
Publication Year
2013
Abstract
The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. Using lipase B from Candida antarctica as a reporter, the relative strength of GAL10 promoter (PGAL10) in Δgal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters, which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. PGAL10 in the Δgal80 mutant showed 0.8-fold (ADH1), fourfold (PDC1), and 50-fold (PGK) in promoter strength.
Keyword
Δgal80Anaerobic alcoholic fermentationGAL10 promoterSaccharomyces cerevisiae
ISSN
1567-1356
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1567-1364.2012.12009.x
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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