Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir

Abstract

A Gram-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, CH15-1T, was isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the lateblooming period of cyanobacteria. Strain CH15-1T grew optimally at pH 7.0 and 30 6C. A phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain CH15-1T belongs to the genus Arenimonas with the similarity range from 92.6-97.4% and is closely related to Arenimonas oryziterrae YC6267T (97.4%), Arenimonas composti TR7-09T (95.4%), Arenimonas metalli CF5-1T (94.7%), Arenimonas malthae CC-JY-1T (94.6%) and Arenimonas donghaensis HO3-R19T (92.6%). However, the DNA-DNA hybridization between strain CH15-1T and the closest strain, Arenimonas oryziterrae YC6267T, was 8.9-12.9%. The DNA G+C content was 63.9 mol% compared to A. oryziterrae YC626T, 65.8 mol%. Strain CH15-1T included Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. The major fatty acids (.5%) were iso-C15: 0, iso-C16: 0, iso-C14: 0, iso-C11: 0 3-OH, iso-C17: 0 and summed feature 9 (iso-C17: 1ω9c and/or C16: 0 10-methyl). On the basis of phylogenetic, phenotypic and genetic data, strain CH15-1T was classified in the genus Arenimonas as a member of a novel species, for which the name Arenimonas daechungensis sp. nov. is proposed. The type strain is CH15-1T (5KCTC 23553T5DSM 24763T).