pH-dependent structural change of the extracellular sensor domain of the DraK histidine kinase from Streptomyces coelicolor = 스트렙토마이세스 실리칼라의 DraK 히스티딘 카이나아제의 세포외 센서도메인의 pH 의존성 구조변화

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Title
pH-dependent structural change of the extracellular sensor domain of the DraK histidine kinase from Streptomyces coelicolor = 스트렙토마이세스 실리칼라의 DraK 히스티딘 카이나아제의 세포외 센서도메인의 pH 의존성 구조변화
Author(s)
K J Yeo; Eun Hye Kim; E Hwang; Y H Han; Y Eo; H J Kim; Oh Suk KwonYoung-Soo Hong; C Cheong; H K Cheong
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 431, no. 3, pp. 554-559
Publication Year
2013
Abstract
Recently, the DraR/DraK (Sco3063/Sco3062) two-component system (TCS) of Streptomyces coelicolor has been reported to be involved in the differential regulation of antibiotic biosynthesis. However, it has not been shown that under which conditions and how the DraR/DraK TCS is activated to initiate the signal transduction process. Therefore, to understand the sensing mechanism, structural study of the sensory domain of DraK is highly required. Here, we report the biochemical and biophysical properties of the extracellular sensory domain (ESD) of DraK. We observed a reversible pH-dependent conformational change of the ESD in a pH range of 2.5-10. Size-exclusion chromatography and AUC (analytical ultracentrifugation) data indicated that the ESD is predominantly monomeric in solution and exists in equilibrium between monomer and dimer states in acidic condition. Using NMR (nuclear magnetic resonance) and CD (circular dichroism) spectroscopy, our findings suggest that the structure of the ESD at low pH is more structured than that at high pH. In particular, the glutamate at position 83 is an important residue for the pH-dependent conformational change. These results suggest that this pH-dependent conformational change of ESD may be involved in signal transduction process of DraR/DraK TCS.
Keyword
DraKPH-dependent conformational changePH-sensitive histidine kinaseSCO3062Sensory domainStreptomyces coelicolorTwo-component system
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2013.01.018
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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