Effect of porcine follicular fluid on donor cell characteristics and quality of porcine cloned blastocysts

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dc.contributor.authorD J Kwon-
dc.contributor.authorK B Oh-
dc.contributor.authorS A Ock-
dc.contributor.authorJeong Woong Lee-
dc.contributor.authorS S Lee-
dc.contributor.authorJ K Park-
dc.contributor.authorW K Chang-
dc.contributor.authorS Hwang-
dc.date.accessioned2017-04-19T09:36:57Z-
dc.date.available2017-04-19T09:36:57Z-
dc.date.issued2012-
dc.identifier.issnI000-0006-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11219-
dc.description.abstractThis study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF (pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group (p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and quality of the cloned blastocyst.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEffect of porcine follicular fluid on donor cell characteristics and quality of porcine cloned blastocysts-
dc.title.alternativeEffect of porcine follicular fluid on donor cell characteristics and quality of porcine cloned blastocysts-
dc.typeArticle-
dc.citation.titleReproductive & Developmental Biology-
dc.citation.number4-
dc.citation.endPage254-
dc.citation.startPage249-
dc.citation.volume36-
dc.contributor.affiliatedAuthorJeong Woong Lee-
dc.contributor.alternativeName권대진-
dc.contributor.alternativeName오건봉-
dc.contributor.alternativeName옥선아-
dc.contributor.alternativeName이정웅-
dc.contributor.alternativeName이성수-
dc.contributor.alternativeName박진기-
dc.contributor.alternativeName장원경-
dc.contributor.alternativeName황성수-
dc.identifier.bibliographicCitationReproductive & Developmental Biology, vol. 36, no. 4, pp. 249-254-
dc.subject.keywordPorcine follicular fluid-
dc.subject.keywordAmino acids-
dc.subject.keywordCell cycle-
dc.subject.keywordCloned embryo development-
dc.subject.localPorcine follicular fluid-
dc.subject.localAmino acid-
dc.subject.localamino acid-
dc.subject.localAmino acids-
dc.subject.localcell cycle-
dc.subject.localCell cycle-
dc.subject.localCloned embryo development-
dc.description.journalClassN-
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Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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