Genetic landscape of open chromatin in yeast

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dc.contributor.authorK Lee-
dc.contributor.authorSang Cheol Kim-
dc.contributor.authorI Jung-
dc.contributor.authorK Kim-
dc.contributor.authorJ Seo-
dc.contributor.authorH S Lee-
dc.contributor.authorG K Bogu-
dc.contributor.authorD Kim-
dc.contributor.authorS Lee-
dc.contributor.authorByungUk Lee-
dc.contributor.authorJ K Choi-
dc.date.accessioned2017-04-19T09:37:14Z-
dc.date.available2017-04-19T09:37:14Z-
dc.date.issued2013-
dc.identifier.issn15537390-
dc.identifier.uri10.1371/journal.pgen.1003229ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11238-
dc.description.abstractChromatin regulation underlies a variety of DNA metabolism processes, including transcription, recombination, repair, and replication. To perform a quantitative genetic analysis of chromatin accessibility, we obtained open chromatin profiles across 96 genetically different yeast strains by FAIRE (formaldehyde-assisted isolation of regulatory elements) assay followed by sequencing. While 5~10% of open chromatin region (OCRs) were significantly affected by variations in their underlying DNA sequences, subtelomeric areas as well as gene-rich and gene-poor regions displayed high levels of sequence-independent variation. We performed quantitative trait loci (QTL) mapping using the FAIRE signal for each OCR as a quantitative trait. While individual OCRs were associated with a handful of specific genetic markers, gene expression levels were associated with many regulatory loci. We found multi-target trans-loci responsible for a very large number of OCRs, which seemed to reflect the widespread influence of certain chromatin regulators. Such regulatory hotspots were enriched for known regulatory functions, such as recombinational DNA repair, telomere replication, and general transcription control. The OCRs associated with these multi-target trans-loci coincided with recombination hotspots, telomeres, and gene-rich regions according to the function of the associated regulators. Our findings provide a global quantitative picture of the genetic architecture of chromatin regulation.-
dc.publisherPublic Library of Science-
dc.titleGenetic landscape of open chromatin in yeast-
dc.title.alternativeGenetic landscape of open chromatin in yeast-
dc.typeArticle-
dc.citation.titlePLoS Genetics-
dc.citation.number2-
dc.citation.endPage1003229-
dc.citation.startPage1003229-
dc.citation.volume9-
dc.contributor.affiliatedAuthorByungUk Lee-
dc.contributor.alternativeName이기백-
dc.contributor.alternativeName김상철-
dc.contributor.alternativeName정인경-
dc.contributor.alternativeName김권일-
dc.contributor.alternativeName서정민-
dc.contributor.alternativeName이헌식-
dc.contributor.alternativeNameBogu-
dc.contributor.alternativeName김동섭-
dc.contributor.alternativeName이상혁-
dc.contributor.alternativeName이병욱-
dc.contributor.alternativeName최정균-
dc.identifier.bibliographicCitationPLoS Genetics, vol. 9, no. 2, pp. 1003229-1003229-
dc.identifier.doi10.1371/journal.pgen.1003229-
dc.description.journalClassY-
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