A rice immunophilin gene, OsFKBP16-3, confers tolerance to environmental stress in Arabidopsis and rice

Cited 11 time in scopus
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Title
A rice immunophilin gene, OsFKBP16-3, confers tolerance to environmental stress in Arabidopsis and rice
Author(s)
Hyun Ji Park; Sang Sook Lee; Young Nim You; Dae Hwa Yoon; B G Kim; J C Ahn; Hye Sun Cho
Bibliographic Citation
International Journal of Molecular Sciences, vol. 14, no. 3, pp. 5899-5919
Publication Year
2013
Abstract
The putative thylakoid lumen immunophilin, FKBP16-3, has not yet been characterized, although this protein is known to be regulated by thioredoxin and possesses a well-conserved CxxxC motif in photosynthetic organisms. Here, we characterized rice OsFKBP16-3 and examined the role of this gene in the regulation of abiotic stress in plants. FKBP16-3s are well conserved in eukaryotic photosynthetic organisms, including the presence of a unique disulfide-forming CxxxC motif in their N-terminal regions. OsFKBP16-3 was mainly expressed in rice leaf tissues and was upregulated by various abiotic stresses, including salt, drought, high light, hydrogen peroxide, heat and methyl viologen. The chloroplast localization of OsFKBP16-3-GFP was confirmed through the transient expression of OsFKBP16-3 in Nicotiana benthamiana leaves. Transgenic Arabidopsis and transgenic rice plants that constitutively expressed OsFKBP16-3 exhibited increased tolerance to salinity, drought and oxidative stresses, but showed no change in growth or phenotype, compared with vector control plants, when grown under non-stressed conditions. This is the first report to demonstrate the potential role of FKBP16-3 in the environmental stress response, which may be regulated by a redox relay process in the thylakoid lumen, suggesting that artificial regulation of FKBP16-3 expression is a candidate for stress-tolerant crop breeding.
Keyword
Environmental stress toleranceFK506 binding proteinFKBP16-3Thylakoid lumen
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms14035899
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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