DC Field | Value | Language |
---|---|---|
dc.contributor.author | S S Sekhon | - |
dc.contributor.author | H Kaur | - |
dc.contributor.author | T Dutta | - |
dc.contributor.author | K Singh | - |
dc.contributor.author | S Kumari | - |
dc.contributor.author | Sunghyun Kang | - |
dc.contributor.author | Sung Goo Park | - |
dc.contributor.author | Byoung Chul Park | - |
dc.contributor.author | Dae Gwin Jeong | - |
dc.contributor.author | A Pareek | - |
dc.contributor.author | Eui-jeon Woo | - |
dc.contributor.author | P Singh | - |
dc.contributor.author | Tae-Sung Yoon | - |
dc.date.accessioned | 2017-04-19T09:37:50Z | - |
dc.date.available | 2017-04-19T09:37:50Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Acta Crystallographica. Section D, Biological Crystallography,69,4,555,563 | ko |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | 10.1107/S0907444912051529 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/11256 | - |
dc.description.abstract | Cyclophilins belong to a family of proteins that bind to the immunosuppressive drug cyclosporin A (CsA). Several members of this protein family catalyze the cis-trans isomerization of peptide bonds preceding prolyl residues. The present study describes the biochemical and structural characteristics of a cytosolic cyclophilin (TaCypA-1) cloned from wheat (Triticum aestivum L.). Purified TaCypA-1 expressed in Escherichia coli showed peptidyl-prolyl cis-trans isomerase activity, which was inhibited by CsA with an inhibition constant of 78.3nM. The specific activity and catalytic efficiency (k cat/K m) of the purified TaCypA-1 were 99.06 ± 0.13nmols -1mg-1 and 2.32 × 105 M -1s-1, respectively. The structures of apo TaCypA-1 and the TaCypA-1-CsA complex were determined at 1.25 and 1.20A resolution, respectively, using X-ray diffraction. Binding of CsA to the active site of TaCypA-1 did not result in any significant conformational change in the apo TaCypA-1 structure. This is consistent with the crystal structure of the human cyclophilin D-CsA complex reported at 0.96A resolution. The TaCypA-1 structure revealed the presence of a divergent loop of seven amino acids 48KSGKPLH54 which is a characteristic feature of plant cyclophilins. This study is the first to elucidate the structure of an enzymatically active plant cyclophilin which shows peptidyl-prolyl cis-trans isomerase activity and the presence of a divergent loop. | - |
dc.publisher | Int Union Crystallography | - |
dc.title | Structural and biochemical characterization of the cytosolic wheat cyclophilin TaCypA-1 = 빵밀유래 시토졸 시클로필린TaCypA-1의 구조 및 생화학적 특성 | - |
dc.title.alternative | Structural and biochemical characterization of the cytosolic wheat cyclophilin TaCypA-1 | - |
dc.type | Article | - |
dc.citation.title | Acta Crystallographica Section D-Biological Crystallography | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 563 | - |
dc.citation.startPage | 555 | - |
dc.citation.volume | 69 | - |
dc.contributor.affiliatedAuthor | Sunghyun Kang | - |
dc.contributor.affiliatedAuthor | Sung Goo Park | - |
dc.contributor.affiliatedAuthor | Byoung Chul Park | - |
dc.contributor.affiliatedAuthor | Dae Gwin Jeong | - |
dc.contributor.affiliatedAuthor | Eui-jeon Woo | - |
dc.contributor.affiliatedAuthor | Tae-Sung Yoon | - |
dc.contributor.alternativeName | Sekhon | - |
dc.contributor.alternativeName | Kaur | - |
dc.contributor.alternativeName | Dutta | - |
dc.contributor.alternativeName | Singh | - |
dc.contributor.alternativeName | Kumari | - |
dc.contributor.alternativeName | 강성현 | - |
dc.contributor.alternativeName | 박성구 | - |
dc.contributor.alternativeName | 박병철 | - |
dc.contributor.alternativeName | 정대균 | - |
dc.contributor.alternativeName | Pareek | - |
dc.contributor.alternativeName | 우의전 | - |
dc.contributor.alternativeName | Singh | - |
dc.contributor.alternativeName | 윤태성 | - |
dc.identifier.bibliographicCitation | Acta Crystallographica Section D-Biological Crystallography, vol. 69, no. 4, pp. 555-563 | - |
dc.identifier.doi | 10.1107/S0907444912051529 | - |
dc.subject.keyword | cyclophilins | - |
dc.subject.keyword | peptidyl-prolyl cis-trans isomerase | - |
dc.subject.keyword | Triticum aestivum | - |
dc.subject.local | Cyclophilin | - |
dc.subject.local | cyclophilins | - |
dc.subject.local | peptidyl-prolyl cis-trans isomerase | - |
dc.subject.local | peptidyl-prolyl cis/trans-isomerase | - |
dc.subject.local | Peptidyl prolyl cis/trans isomerase | - |
dc.subject.local | Triticum aestivum L. | - |
dc.subject.local | Triticum aestivum L | - |
dc.subject.local | Triticum aestivum | - |
dc.description.journalClass | Y | - |
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