Gaetbulibacter lutimaris sp. nov., isolated from a tidal flat sediment

Abstract

A Gram-stain-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, D1-y4T, was isolated from a tidal flat sediment of the South Sea in South Korea and subjected to a polyphasic study. Strain D1-y4T grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain D1-y4T belonged to the genus Gaetbulibacter, joining the type strain of Gaetbulibacter marinus, with which it exhibited 97.8 % similarity. Sequence similarities to Gaetbulibacter saemankumensis SMK-12T and Gaetbulibacter aestuarii KYW382T were 96.5 and 96.2 %, respectively. Strain D1-y4T contained MK-6 as the predominant menaquionone and iso-C15: 0, iso-C15: 115: 1 G and anteiso-C15: 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain D1-y4T was 34.6 mol% and its mean DNA-DNA relatedness value with G. marinus KCTC 23046T was 7 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain D1-y4T is distinguishable from the three recognized Gaetbulibacter species. On the basis of the data presented here, strain D1-y4T is considered to represent a novel species of the genus Gaetbulibacter, for which the name Gaetbulibacter lutimaris sp. nov. is proposed. The type strain is D1-y4T (= KCTC 23716T = CCUG 61504T).