DC Field | Value | Language |
---|---|---|
dc.contributor.author | Y P Hwang | - |
dc.contributor.author | H G Kim | - |
dc.contributor.author | J H Choi | - |
dc.contributor.author | M T Do | - |
dc.contributor.author | T P Tran | - |
dc.contributor.author | Hyo Kon Chun | - |
dc.contributor.author | Y C Chung | - |
dc.contributor.author | T C Jeong | - |
dc.contributor.author | H G Jeong | - |
dc.date.accessioned | 2017-04-19T09:38:32Z | - |
dc.date.available | 2017-04-19T09:38:32Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1613-4125 | - |
dc.identifier.uri | 10.1002/mnfr.201200529 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/11269 | - |
dc.description.abstract | Scope: Increasing evidence indicates that polyphenols may protect against metabolic disease through activating AMP-activated protein kinase (AMPK). The aims of our study were to provide new data on the molecular mechanism(s) underlying the role of the phenolic compound, 3-caffeoyl, 4-dihydrocaffeoylquinic acid (CDCQ) from Salicornia herbacea, in the prevention of high glucose-induced lipogenesis in human HepG2 cells. Methods and results: Nile red staining assays were used to demonstrate lipid accumulation in the cells. Expression of sterol regulatory element-binding protein-1c (SREBP-1c) and fatty acid synthase (FAS) gene at the levels of promoter activity, mRNA, and protein was demonstrated using transient transfection assays, quantitative RT-PCR, and Western blot analyses, respectively. We found that CDCQ suppressed high glucose-induced lipid accumulation in HepG2 cells. CDCQ strongly inhibited high glucose-induced FAS expression by modulating SREBP-1c activation. Moreover, the use of both a specific inhibitor and liver kinase B1 (LKB1)-siRNA transfected HepG2 cells showed that CDCQ activated AMPK via silent information regulator T1 (SIRT1) or LKB1 in HepG2 cells. Conclusion: These results indicate that CDCQ prevented lipid accumulation by blocking the expression of SREBP-1c and FAS through LKB1/SIRT1 and AMPK activation in HepG2 cells, suggesting that CDCQ plays a potential role in the prevention of lipogenesis by AMPK activation. | - |
dc.publisher | Wiley | - |
dc.title | 3-Caffeoyl, 4-dihydrocaffeoylquinic acid from Salicornia herbacea attenuates high glucose-induced hepatic lipogenesis in human HepG2 cells through activation of the liver kinase B1 and silent information regulator T1/AMPK-dependent pathway | - |
dc.title.alternative | 3-Caffeoyl, 4-dihydrocaffeoylquinic acid from Salicornia herbacea attenuates high glucose-induced hepatic lipogenesis in human HepG2 cells through activation of the liver kinase B1 and silent information regulator T1/AMPK-dependent pathway | - |
dc.type | Article | - |
dc.citation.title | Molecular Nutrition & Food Research | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 482 | - |
dc.citation.startPage | 471 | - |
dc.citation.volume | 57 | - |
dc.contributor.affiliatedAuthor | Hyo Kon Chun | - |
dc.contributor.alternativeName | 황용필 | - |
dc.contributor.alternativeName | 김형균 | - |
dc.contributor.alternativeName | 최재호 | - |
dc.contributor.alternativeName | Do | - |
dc.contributor.alternativeName | Tran | - |
dc.contributor.alternativeName | 전효곤 | - |
dc.contributor.alternativeName | 정영철 | - |
dc.contributor.alternativeName | 정태천 | - |
dc.contributor.alternativeName | 정혜광 | - |
dc.identifier.bibliographicCitation | Molecular Nutrition & Food Research, vol. 57, no. 3, pp. 471-482 | - |
dc.identifier.doi | 10.1002/mnfr.201200529 | - |
dc.subject.keyword | 3-Caffeoyl, 4-dihydrocaffeoylquinic acid | - |
dc.subject.keyword | AMPK | - |
dc.subject.keyword | High glucose | - |
dc.subject.keyword | Lipogenesis | - |
dc.subject.keyword | LKB1 | - |
dc.subject.local | 3-Caffeoyl, 4-dihydrocaffeoylquinic acid | - |
dc.subject.local | 3-caffeoyl-4-dihydrocaffeoyl quinic acid | - |
dc.subject.local | AMPK | - |
dc.subject.local | High glucose | - |
dc.subject.local | lipogenesis | - |
dc.subject.local | Lipogenesis | - |
dc.subject.local | LKB1 | - |
dc.description.journalClass | Y | - |
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