The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae

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Title
The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae
Author(s)
Baek Rock Oh; Won-Kyung Hong; Sun-Yeon Heo; M H Joe; Jeong-Woo SeoChul Ho Kim
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 40, no. 2, pp. 227-233
Publication Year
2013
Abstract
Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l-1, when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l-1 under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.
Keyword
Aldehyde/alcohol dehydrogenase (AdhE)EthanolGlycerolKlebsiella pneumoniae
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10295-012-1224-8
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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