DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung Ho Park | - |
dc.contributor.author | S Yoshizumi | - |
dc.contributor.author | Y Yamaguchi | - |
dc.contributor.author | K P Wu | - |
dc.contributor.author | M Inouye | - |
dc.date.accessioned | 2017-04-19T09:38:53Z | - |
dc.date.available | 2017-04-19T09:38:53Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0887-3585 | - |
dc.identifier.uri | 10.1002/prot.24246 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/11274 | - |
dc.description.abstract | MazF is an mRNA interferase that cleaves mRNAs at a specific RNA sequence. MazF from E. coli (MazF-ec) cleaves RNA at A and CA. To date, a large number of MazF homologs that cleave RNA at specific three- to seven-base sequences have been identified from bacteria to archaea. MazF-ec forms a dimer, in which the interface between the two subunits is known to be the RNA substrate-binding site. Here, we investigated the role of the two loops in MazF-ec, which are closely associated with the interface of the MazF-ec dimer. We examined whether exchanging the loop regions of MazF-ec with those from other MazF homologs, such as MazF from Myxococcus xanthus (MazF-mx) and MazF from Mycobacterium tuberculosis (MazF-mt3), affects RNA cleavage specificity. We found that exchanging loop 2 of MazF-ec with loop 2 regions from either MazF-mx or MazF-mt3 created a new cleavage sequence at (A/U)(A/U)AA and C in addition to the original cleavage site, A and CA, whereas exchanging loop 1 did not alter cleavage specificity. Intriguingly, exchange of loop 2 with 8 or 12 consecutive Gly residues also resulted in a new RNA cleavage site at (A/U)(A/U)AA and C. The present study suggests a method for expanding the RNA cleavage repertoire of mRNA interferases, which is crucial for potential use in the regulation of specific gene expression and for biotechnological applications. | - |
dc.publisher | Wiley | - |
dc.title | ACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase | - |
dc.title.alternative | ACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase | - |
dc.type | Article | - |
dc.citation.title | Proteins-Structure Function and Bioinformatics | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 883 | - |
dc.citation.startPage | 874 | - |
dc.citation.volume | 81 | - |
dc.contributor.affiliatedAuthor | Jung Ho Park | - |
dc.contributor.alternativeName | 박정호 | - |
dc.contributor.alternativeName | Yoshizumi | - |
dc.contributor.alternativeName | Yamaguchi | - |
dc.contributor.alternativeName | Wu | - |
dc.contributor.alternativeName | Inouye | - |
dc.identifier.bibliographicCitation | Proteins-Structure Function and Bioinformatics, vol. 81, no. 5, pp. 874-883 | - |
dc.identifier.doi | 10.1002/prot.24246 | - |
dc.subject.keyword | Chimeric proteins | - |
dc.subject.keyword | MazE-MazF | - |
dc.subject.keyword | Sequence-specific endoribonucleases | - |
dc.subject.keyword | TA systems | - |
dc.subject.local | Chimeric proteins | - |
dc.subject.local | MazE-MazF | - |
dc.subject.local | Sequence-specific endoribonucleases | - |
dc.subject.local | TA systems | - |
dc.subject.local | TA system | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.