ACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase

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dc.contributor.authorJung Ho Park-
dc.contributor.authorS Yoshizumi-
dc.contributor.authorY Yamaguchi-
dc.contributor.authorK P Wu-
dc.contributor.authorM Inouye-
dc.date.accessioned2017-04-19T09:38:53Z-
dc.date.available2017-04-19T09:38:53Z-
dc.date.issued2013-
dc.identifier.issn0887-3585-
dc.identifier.uri10.1002/prot.24246ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11274-
dc.description.abstractMazF is an mRNA interferase that cleaves mRNAs at a specific RNA sequence. MazF from E. coli (MazF-ec) cleaves RNA at A and CA. To date, a large number of MazF homologs that cleave RNA at specific three- to seven-base sequences have been identified from bacteria to archaea. MazF-ec forms a dimer, in which the interface between the two subunits is known to be the RNA substrate-binding site. Here, we investigated the role of the two loops in MazF-ec, which are closely associated with the interface of the MazF-ec dimer. We examined whether exchanging the loop regions of MazF-ec with those from other MazF homologs, such as MazF from Myxococcus xanthus (MazF-mx) and MazF from Mycobacterium tuberculosis (MazF-mt3), affects RNA cleavage specificity. We found that exchanging loop 2 of MazF-ec with loop 2 regions from either MazF-mx or MazF-mt3 created a new cleavage sequence at (A/U)(A/U)AA and C in addition to the original cleavage site, A and CA, whereas exchanging loop 1 did not alter cleavage specificity. Intriguingly, exchange of loop 2 with 8 or 12 consecutive Gly residues also resulted in a new RNA cleavage site at (A/U)(A/U)AA and C. The present study suggests a method for expanding the RNA cleavage repertoire of mRNA interferases, which is crucial for potential use in the regulation of specific gene expression and for biotechnological applications.-
dc.publisherWiley-
dc.titleACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase-
dc.title.alternativeACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase-
dc.typeArticle-
dc.citation.titleProteins-Structure Function and Bioinformatics-
dc.citation.number5-
dc.citation.endPage883-
dc.citation.startPage874-
dc.citation.volume81-
dc.contributor.affiliatedAuthorJung Ho Park-
dc.contributor.alternativeName박정호-
dc.contributor.alternativeNameYoshizumi-
dc.contributor.alternativeNameYamaguchi-
dc.contributor.alternativeNameWu-
dc.contributor.alternativeNameInouye-
dc.identifier.bibliographicCitationProteins-Structure Function and Bioinformatics, vol. 81, no. 5, pp. 874-883-
dc.identifier.doi10.1002/prot.24246-
dc.subject.keywordChimeric proteins-
dc.subject.keywordMazE-MazF-
dc.subject.keywordSequence-specific endoribonucleases-
dc.subject.keywordTA systems-
dc.subject.localChimeric proteins-
dc.subject.localMazE-MazF-
dc.subject.localSequence-specific endoribonucleases-
dc.subject.localTA systems-
dc.subject.localTA system-
dc.description.journalClassY-
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Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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