Lipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii

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dc.contributor.authorR Ramanan-
dc.contributor.authorByung-Hyuk Kim-
dc.contributor.authorDae-Hyun Cho-
dc.contributor.authorSo Ra Ko-
dc.contributor.authorHee-Mock Oh-
dc.contributor.authorHee-Sik Kim-
dc.date.accessioned2017-04-19T09:38:54Z-
dc.date.available2017-04-19T09:38:54Z-
dc.date.issued2013-
dc.identifier.issn0014-5793-
dc.identifier.uri10.1016/j.febslet.2012.12.020ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11275-
dc.description.abstractPhenotypic and genotypic changes in Chlamydomonas reinhardtii BafJ5, a starchless mutant, with respect to lipid metabolism was studied in different trophic states under nitrogen (N) sufficient and limited conditions. Interestingly, cellular lipid content increased linearly with input acetate concentration with highest lipid content (∼42%) under nitrogen limitation and mixotrophic state. RT-qPCR studies indicate that key fatty acid biosynthesis genes are down-regulated under N limitation but not under mixotrophic state, whereas, ACS2, encoding Acetyl-CoA synthetase, and DGTT4, encoding Diacylglycerol O-acyltransferase, are up-regulated under all conditions. These results collectively indicate that acetate is the limiting factor and central molecule in lipid droplet synthesis. The study also provides further evidence of the presence of a chloroplast pathway for triacylglycerol synthesis in microalgae.-
dc.publisherWiley-
dc.titleLipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii-
dc.title.alternativeLipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii-
dc.typeArticle-
dc.citation.titleFEBS Letters-
dc.citation.number4-
dc.citation.endPage377-
dc.citation.startPage370-
dc.citation.volume587-
dc.contributor.affiliatedAuthorByung-Hyuk Kim-
dc.contributor.affiliatedAuthorDae-Hyun Cho-
dc.contributor.affiliatedAuthorSo Ra Ko-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.affiliatedAuthorHee-Sik Kim-
dc.contributor.alternativeNameRamanan-
dc.contributor.alternativeName김병혁-
dc.contributor.alternativeName조대현-
dc.contributor.alternativeName고소라-
dc.contributor.alternativeName오희목-
dc.contributor.alternativeName김희식-
dc.identifier.bibliographicCitationFEBS Letters, vol. 587, no. 4, pp. 370-377-
dc.identifier.doi10.1016/j.febslet.2012.12.020-
dc.subject.keywordChlamydomonas reinhardtii-
dc.subject.keywordChloroplast-
dc.subject.keywordFatty acid biosynthesis-
dc.subject.keywordGene expression-
dc.subject.keywordLipid droplet-
dc.subject.localChlamydomonas reinhardtii-
dc.subject.localchlamydomonas reinhardtii-
dc.subject.localchloroplast-
dc.subject.localChloroplast-
dc.subject.localFatty acid biosynthesis-
dc.subject.localfatty acid biosynthesis-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.locallipid droplet-
dc.subject.localLipid droplet-
dc.subject.localLipid droplets-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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