Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation

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Title
Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation
Author(s)
Baek Rock OhJeong-Woo SeoSun-Yeon Heo; L H Luo; Won-Kyung Hong; D H Park; Chul Ho Kim
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 36, no. 6, pp. 757-763
Publication Year
2013
Abstract
In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 PDE20 promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.
Keyword
1,3-PropanediolBy-product formationConstitutive expressionGlycerolKlebsiella pneumoniae
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-013-0901-y
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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