Induction of rat liver tumor using the Sleeping Beauty transposon and electroporation

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dc.contributor.authorJ S Park-
dc.contributor.authorB H Kim-
dc.contributor.authorSung Goo Park-
dc.contributor.authorS Y Jung-
dc.contributor.authorD H Lee-
dc.contributor.authorW C Son-
dc.date.accessioned2017-04-19T09:39:36Z-
dc.date.available2017-04-19T09:39:36Z-
dc.date.issued2013-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2013.03.119ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11320-
dc.description.abstractThe Sleeping Beauty (SB) transposon system has been receiving much attention as a gene transfer method of choice since it allows permanent gene expression after insertion into the host chromosome. However, low transposition frequency in higher eukaryotes limits its use in commonly-used mammalian species. Researchers have therefore attempted to modify gene delivery and expression to overcome this limitation. In mouse liver, tumor induction using SB introduced by the hydrodynamic method has been successfully accomplished. Liver tumor in rat models using SB could also be of great use; however, dose of DNA, injection volume, rate of injection and achieving back pressure limit the use of the hydrodynamics-based gene delivery. In the present study, we combined the electroporation, a relatively simple and easy gene delivery method, with the SB transposon system and as a result successfully induced tumor in rat liver by directly injecting the c-Myc, HRAS and shp53 genes. The tumor phenotype was determined as a sarcomatoid carcinoma. To our knowledge, this is the first demonstration of induction of tumor in the rat liver using the electroporation-enhanced SB transposon system.-
dc.publisherElsevier-
dc.titleInduction of rat liver tumor using the Sleeping Beauty transposon and electroporation-
dc.title.alternativeInduction of rat liver tumor using the Sleeping Beauty transposon and electroporation-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number3-
dc.citation.endPage593-
dc.citation.startPage589-
dc.citation.volume434-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.alternativeName박준신-
dc.contributor.alternativeName김배환-
dc.contributor.alternativeName박성구-
dc.contributor.alternativeName정선영-
dc.contributor.alternativeName이도희-
dc.contributor.alternativeName손우찬-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 434, no. 3, pp. 589-593-
dc.identifier.doi10.1016/j.bbrc.2013.03.119-
dc.subject.keywordElectroporation-
dc.subject.keywordLiver tumor-
dc.subject.keywordRat-
dc.subject.keywordSleeping Beauty transposon-
dc.subject.localElectroporation-
dc.subject.localelectroporation-
dc.subject.localLiver tumor-
dc.subject.localRat-
dc.subject.localrat-
dc.subject.localRats-
dc.subject.localrats-
dc.subject.localRat.-
dc.subject.localSleeping beauty transposon-
dc.subject.localSleeping Beauty transposon-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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