Engineered heterologous FPP synthases-mediated Z,E-FPP synthesis in E. coli = 외래 FPP synthase를 활용한 대장균에서의 Z,E-FPP의 생합성

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Title
Engineered heterologous FPP synthases-mediated Z,E-FPP synthesis in E. coli = 외래 FPP synthase를 활용한 대장균에서의 Z,E-FPP의 생합성
Author(s)
C Wang; J Zhou; H J Jang; S H Yoon; J Y Kim; Seung Goo Lee; Eui Sung Choi; S W Kim
Bibliographic Citation
Metabolic Engineering, vol. 18, no. 1, pp. 53-59
Publication Year
2013
Abstract
Production of Z-type farnesyl diphosphate (FPP) has not been reported in Escherichia coli. Here we present the fusion enzyme (ILRv) of E. coli E,. E-FPP synthase (IspA) and Mycobacterium tuberculosis Z,. E-FPP synthase (Rv1086), which can produce primarily Z,. E-FPP rather than E,. E-FPP, the predominant stereoisomer found in most organisms. Z,. E-farnesol (FOH) was produced from E. coli harboring the bottom portion of the MVA pathway and the fusion FPP synthase (ILRv) at a titer of 115.6. mg/L in 2. YT medium containing 1% (v/v) glycerol as a carbon source and 5. mM mevalonate. The Z,E-FOH production was improved by 15-fold, compared with 7.7. mg/L obtained from the co-overexpression of separate IspA and Rv1086. The Z,. E-FPP was not metabolized in native metabolic pathways of E. coli. It would be of interest to produce Z,. E-FPP derived sesquiterpenes from recombinant E. coli due to no loss of Z,. E-FPP substrate in endogenous metabolism of the host strain.
Keyword
FPP synthaseProtein fusionSesquiterpeneZ,E-FarnesolZ,E-FPP
ISSN
1096-7176
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ymben.2013.04.002
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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