Development of episomal vectors carrying a nourseothricin-resistance marker for use in minimal media for Schizosaccharomyces pombe = 분열효모의 최소배지에서 활용 가능한 노르세오트리신 저항마커를 가지는 벡터 개발

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Title
Development of episomal vectors carrying a nourseothricin-resistance marker for use in minimal media for Schizosaccharomyces pombe = 분열효모의 최소배지에서 활용 가능한 노르세오트리신 저항마커를 가지는 벡터 개발
Author(s)
Jiwon AhnMi Sun Won; M L Kyun; Yong Sung Kim; Cho-Rok Jung; Dong-Soo Im; K B Song; Kyung-Sook Chung
Bibliographic Citation
Yeast, vol. 30, no. 6, pp. 219-227
Publication Year
2013
Abstract
In the post-genomic era, an immediate challenge is to assign biological functions to novel proteins encoded by the genome. This challenge requires the use of a simple organism as a genetic tool and a range of new high-throughput techniques. Schizosacchromyces pombe is a powerful model organism used to investigate disease-related genes and provides useful tools for the functional analysis of heterologous genes. To expand the current array of experimental tools, we constructed two series of Sz. pombe expression vectors, i.e. general and Gateway vectors, containing nourseothricin-resistance markers. Vectors carrying nourseothricin-resistance markers possess advantages in that they do not limit the parental strains with auxotrophic mutations with respect to availability for use in clone selection and can be used together with vectors carrying nutrient markers in minimal media. We modified the pSLF173, pSLF273 and pSLF373 vectors carrying a triple haemagglutinin epitope (3×HA) and an Ura4 marker. The vectors described here contain the nmt1 promoter with three different episomal expression strengths for proteins fused with 3×HA, EGFP or DsRed at the N-terminus. These vectors represent an important contribution to the genome-wide investigation of multiple heterologous genes and for functional and genetic analysis of novel human genes.
Keyword
Schizosaccharomyces pombeExpression vectorGateway systemMinimal mediaNourseothricin-resistance marker
ISSN
0749-503X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/yea.2955
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
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