Belnapia soli sp. nov., a proteobacterium isolated from grass soil

Abstract

A Gram-negative, aerobic, non-motile, non-spore-forming, cocci-shaped, red-pigmented bacterium, designated strain PB-K8T, was isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the class Alphaproteobacteria, and was most closely related to Belnapia moabensis DSM 16746T and Belnapia rosea DSM 23312T, showing a 16S rRNA gene sequence similarity to the type strains of each species of 98.4 and 97.2 %, respectively. The cells of strain PB-K8T formed red colonies on R2A agar, contained Q-9 as the predominant ubiquinone and included summed feature 3 (C16: 1ω7c C16: 1ω6c), C16: 0, summed feature 8 (C18: 1ω7c/C18: 1ω6c), C18: 1 2-OH and C19: 0 cyclo ω8c as the major fatty acids. The G+C content of the genomic DNA of strain PB-K8T was 72.1 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain PB-K8T represents a novel species of the genus Belnapia, for which the name Belnapia soli sp. nov. is proposed. The type strain is PB-K8T (=KCTC 23765T=JCM 18033T).