Functional display of active tetrameric ß-galactosidase using Bacillus subtilis spore display system

Cited 14 time in scopus
Metadata Downloads
Title
Functional display of active tetrameric ß-galactosidase using Bacillus subtilis spore display system
Author(s)
B Y Hwang; Jae Gu Pan; B G Kim; J H Kim
Bibliographic Citation
Journal of Nanoscience and Nanotechnology, vol. 13, no. 3, pp. 2313-2319
Publication Year
2013
Abstract
For the functional bacterial surface display of active enzyme of multimeric form, which is generally impossible due to molecular assembly of the monomer subunit subsequent to the secretion of displayed target protein outside the cell, a new surface display system based on B. subtilis spore was developed. Using cotE and cotG of B. subtilis as anchoring motives, ß-galactosidase, which is active in tetrameric form, was functionally displayed on the surface of B. subtilis spore. The surface localization of ß-galactosidase was verified by Miller assay of purified spore, protease accessibility test of purified spore, and flow cytometric analysis of spore expressing ß-galactosidase. While B. subtilis spore wall integrity, examined by lysozyme and heat treatments, was affected by the incorporation of CotE-LacZ fusion protein, it was not affected by the incorporation of CotG-lacZ fusion. Heat stability of displayed protein was similar with that of free enzyme.
Keyword
ß-GalactosidaseBacillus subtilisSporeSurface display
ISSN
1533-4880
Publisher
Amer Scientific Publishers
DOI
http://dx.doi.org/10.1166/jnn.2013.6889
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.