Characterization of a squalene synthase from the thraustochytrid microalga Aurantiochytrium sp. KRS101

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Title
Characterization of a squalene synthase from the thraustochytrid microalga Aurantiochytrium sp. KRS101
Author(s)
Won-Kyung Hong; Sun-Yeon Heo; Hye Mi Park; Chul Ho KimJung Hoon Sohn; A Kondo; Jeong-Woo Seo
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 23, no. 6, pp. 759-765
Publication Year
2013
Abstract
The gene encoding squalene synthase (SQS) of the lipidproducing heterotrophic microalga Aurantiochytrium sp. KRS101 was cloned and characterized. The krsSQS gene is 1,551 bp in length and has two exons and one intron. The open reading frame of the gene is 1,164 bp in length, yielding a polypeptide of 387 predicted amino acid residues with a molecular mass of 42.7 kDa. The deduced krsSQS sequence shares at least four conserved regions known to be required for SQS enzymatic activity in other species. The protein, tagged with His6, was expressed into soluble form in Escherichia coli. The purified protein catalyzed the conversion of farnesyl diphosphate to squalene in the presence of NADPH and Mg2+. This is the first report on the characterization of an SQS from a Thraustochytrid microalga
Keyword
AurantiochytriumFunctional characterizationGene analysisSqualene synthase
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1212.12023
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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