Dual-micropillar-based microfluidic platform for single embryonic stem cell-derived neuronal differentiation

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Title
Dual-micropillar-based microfluidic platform for single embryonic stem cell-derived neuronal differentiation
Author(s)
J M Lee; J E Kim; J Borana; Bong Hyun Chung; B G Chung
Bibliographic Citation
Electrophoresis, vol. 34, no. 13, pp. 1931-1938
Publication Year
2013
Abstract
We developed the dual-micropillar-based microfluidic platform to direct embryonic stem (ES) cell fate. 4 × 4 dual-micropillar-based microfluidic platform consisted of 16 circular-shaped outer micropillars and 8 saddle-shaped inner micropillars in which single ES cells were cultured. We hypothesized that dual-micropillar arrays would play an important role in controlling the shear stress and cell docking. Circular-shaped outer micropillars minimized the shear stress, whereas saddle-shaped inner micropillars allowed for docking of individual ES cells. We observed the effect of saddle-shaped inner micropillars on cell docking in response to hydrodynamic resistance. We also demonstrated that ES cells cultured for 6 days within the dual-micropillar-based microfluidic platform differentiated into neural-like cells. Therefore, this dual-micropillar-based microfluidic platform could be a potentially powerful method for screening of lineage commitments of single ES cells.
Keyword
Embryonic stem cellMicrofluidic deviceNeuronal differentiation
ISSN
0173-0835
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/elps.201200578
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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