Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein

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dc.contributor.authorMin Sun Sung-
dc.contributor.authorJi Young Mun-
dc.contributor.authorOh Suk Kwon-
dc.contributor.authorKi Sun Kwon-
dc.contributor.authorDoo-Byoung Oh-
dc.date.accessioned2017-04-19T09:41:27Z-
dc.date.available2017-04-19T09:41:27Z-
dc.date.issued2013-
dc.identifier.issn0006291X-
dc.identifier.uri10.1016/j.bbrc.2013.06.058ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11415-
dc.description.abstractHuman adipose-derived stem cells (hASCs) have great potential as cell sources for the treatment of muscle disorders. To provide a safe method for the myogenic differentiation of hASCs, we engineered the MyoD protein, a key transcription factor for myogenesis. The engineered MyoD (MyoD-IT) was designed to contain the TAT protein transduction domain for cell penetration and the membrane-disrupting INF7 peptide, which is an improved version of the HA2 peptide derived from influenza. MyoD-IT showed greatly improved nuclear targeting ability through an efficient endosomal escape induced by the pH-sensitive membrane disruption of the INF7 peptide. By applying MyoD-IT to a culture, hASCs were efficiently differentiated into long spindle-shaped myogenic cells expressing myosin heavy chains. Moreover, these cells differentiated by an application of MyoD-IT fused to myotubes with high efficiency through co-culturing with mouse C2C12 myoblasts. Because internalized proteins can be degraded in cells without altering the genome, the myogenic differentiation of hASCs using MyoD-IT would be a safe and clinically applicable method.-
dc.publisherElsevier-
dc.titleEfficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein-
dc.title.alternativeEfficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number1-
dc.citation.endPage161-
dc.citation.startPage156-
dc.citation.volume437-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.affiliatedAuthorKi Sun Kwon-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.alternativeName성민선-
dc.contributor.alternativeName문지영-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName권기선-
dc.contributor.alternativeName오두병-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 437, no. 1, pp. 156-161-
dc.identifier.doi10.1016/j.bbrc.2013.06.058-
dc.subject.keywordEndosomal escape-
dc.subject.keywordHuman adipose-derived stem cells-
dc.subject.keywordMyoD-
dc.subject.keywordMyogenic differentiation-
dc.subject.keywordProtein transduction domain-
dc.subject.localEndosomal escape-
dc.subject.localHuman adipose-derived stem cells-
dc.subject.localHuman adipose-derived stem cells (hADSCs)-
dc.subject.localMyoD-
dc.subject.localMyogenic differentiation-
dc.subject.localProtein transduction domain-
dc.subject.localProtein transduction domain (PTD)-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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