Aquihabitans daechungensis gen. nov., sp. nov., an actinobacterium isolated from reservoir water

Abstract

A novel Gram-reaction-positive bacterium, strain CH22-21T, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21T were non-motile, ciliated short rods that formed creamywhite colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H6) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16: 1ω5c, 16:0, 17: 1ω8c and 18: 1ω9c as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order Acidimicrobiales, showing similarity values of <92.3% with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21T could be distinguished from all genera within the order Acidimicrobiales and represented a novel species of a new genus in the family Iamiaceae, for which the name Aquihabitans dachungensis gen. nov., sp. nov. is proposed. The type strain of Aquihabitans dachungensis is CH22-21T (=KCTC 19849T=JCM 17787T).