Identification and characterisation of coumarins from the roots of Angelica dahurica and their inhibitory effects against cholinesterase

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Title
Identification and characterisation of coumarins from the roots of Angelica dahurica and their inhibitory effects against cholinesterase
Author(s)
W D Seo; J Y Kim; Hyung Won Ryu; J H Kim; S I Han; J E Ra; K H Seo; K C Jang; J H Lee
Bibliographic Citation
Journal of Functional Foods, vol. 5, no. 3, pp. 1421-1431
Publication Year
2013
Abstract
The current research was the first to investigate coumarins and their cholinesterase inhibitory activities from Angelica dahurica roots. The ethanol extract of this species (100μg/ml) possessed inhibitory effects against human acetylcholinesterase (hAChE) and butylcholinesterase (BChE) with IC50 values of 26.40 and 14.71μg/ml, respectively. To evaluate the compounds responsible for these activities, the ethanol extract was chromatographed, which yielded ten coumarins, including isoimperatorin (1), imperatorin (2), senbyakangelicol (3), oxypeucedanin (4), byakangelicol (5), t-OMe-oxypeucedanin hydrate (6), t-OMe-byakangelicin (7), angelol H (8), byakangelicin (9), and oxypeucedanin hydrate (10). Among them, coumarin 5, 7, and 9 showed potent inhibition with IC50s of 46.3, 39.0, and 55.4μM (hAChE) as well as 45.2, 25.6, and 42.4μM (BChE), respectively. Moreover, their inhibition modes against two cholinesterases exhibited noncompetitive. The individual coumarin contents were remarkable differences, especially, oxypeucedanin (4) was the most predominant compound (8710.9μg/g), representing approximately 55.0% of the total content.
Keyword
AcetylcholinesteraseAngelica dahuricaButylcholinesteraseCoumarinRootUltra performance liquid chromatography
ISSN
1756-4646
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jff.2013.05.011
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
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