Metabolic signature genes associated with susceptibility to pyruvate kinase, muscle type 2 gene ablation in cancer cells

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dc.contributor.authorYuri Jung-
dc.contributor.authorY J Jang-
dc.contributor.authorMin Ho Kang-
dc.contributor.authorYoung Soo Park-
dc.contributor.authorSu Jin Oh-
dc.contributor.authorDong Chul Lee-
dc.contributor.authorZ Xie-
dc.contributor.authorH S Yoo-
dc.contributor.authorKyung Chan Park-
dc.contributor.authorYoung Il Yeom-
dc.date.accessioned2017-04-19T09:41:30Z-
dc.date.available2017-04-19T09:41:30Z-
dc.date.issued2013-
dc.identifier.issn1016-8478-
dc.identifier.uri10.1007/s10059-013-2319-4ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11431-
dc.description.abstractPyruvate kinase, muscle type 2 (PKM2), is a key factor in the aerobic glycolysis of cancer cells. In our experiments, liver cancer cell lines exhibited a range of sensitivity to PKM2 knockdown-mediated growth inhibition. We speculated that this differential sensitivity is attributable to the variable dependency on glycolysis for the growth of different cell lines. Transcriptome data revealed overexpression of a glucose transporter (GLUT3) and a lactate transporter (MCT4) genes in PKM2 knockdown-sensitive cells. PKM2 knockdown-resistant cells expressed high levels of the lactate dehydrogenase B (LDHB) and glycine decarboxylase (GLDC) genes. Concordant with the gene expression results, PKM2 knockdown-sensitive cells generated high levels of lactate. In addition, ATP production was significantly reduced in the PKM2 knockdown-sensitive cells treated with a glucose analog, indicative of dependency of their cellular energetics on lactate-producing glycolysis. The PKM2 knockdown-resistant cells were further subdivided into less glycolytic and more (glycolysis branch pathway-dependent) glycolytic groups. Our findings collectively support the utility of PKM2 as a therapeutic target for high lactate-producing glycolytic hepatocellular carcinoma (HCC).-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMetabolic signature genes associated with susceptibility to pyruvate kinase, muscle type 2 gene ablation in cancer cells-
dc.title.alternativeMetabolic signature genes associated with susceptibility to pyruvate kinase, muscle type 2 gene ablation in cancer cells-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number4-
dc.citation.endPage341-
dc.citation.startPage335-
dc.citation.volume35-
dc.contributor.affiliatedAuthorYuri Jung-
dc.contributor.affiliatedAuthorMin Ho Kang-
dc.contributor.affiliatedAuthorYoung Soo Park-
dc.contributor.affiliatedAuthorSu Jin Oh-
dc.contributor.affiliatedAuthorDong Chul Lee-
dc.contributor.affiliatedAuthorKyung Chan Park-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.alternativeName정유리-
dc.contributor.alternativeName장예진-
dc.contributor.alternativeName강민호-
dc.contributor.alternativeName박영수-
dc.contributor.alternativeName오수진-
dc.contributor.alternativeName이동철-
dc.contributor.alternativeNameXie-
dc.contributor.alternativeName유향숙-
dc.contributor.alternativeName박경찬-
dc.contributor.alternativeName염영일-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 35, no. 4, pp. 335-341-
dc.identifier.doi10.1007/s10059-013-2319-4-
dc.subject.keywordglycolysis-
dc.subject.keywordglycolysis-dependent-
dc.subject.keywordlactate-
dc.subject.keywordPKM2-
dc.subject.keywordSLC16A3-
dc.subject.localglycolysis-
dc.subject.localGlycolysis-
dc.subject.localglycolysis-dependent-
dc.subject.localLactate-
dc.subject.locallactate-
dc.subject.localPKM2-
dc.subject.localSLC16A3-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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