Efficient adhesion-based plasma membrane isolation for cell surface N-glycan analysis

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dc.contributor.authorJi Young Mun-
dc.contributor.authorK J Lee-
dc.contributor.authorHoon Seo-
dc.contributor.authorMin-Sun Sung-
dc.contributor.authorYee Sook Cho-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorOh Suk Kwon-
dc.contributor.authorDoo-Byoung Oh-
dc.date.accessioned2017-04-19T09:41:43Z-
dc.date.available2017-04-19T09:41:43Z-
dc.date.issued2013-
dc.identifier.issn0003-2700-
dc.identifier.uri10.1021/ac401431uko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11441-
dc.description.abstractGlycans, which decorate cell surfaces, play crucial roles in various physiological events involving cell surface recognition. Despite the importance of surface glycans, most analyses have been performed using total cells or whole membranes rather than plasma membranes due to difficulties related to isolation. In the present study, we employed an adhesion-based method for plasma membrane isolation to analyze N-glycans on cell surfaces. Cells were attached to polylysine-coated glass plates and then ruptured by hypotonic pressure. After washing to remove intracellular organelles, only a plasma membrane fraction remained attached to the plates, as confirmed by fluorescence imaging using organelle-specific probes. The plate was directly treated with trypsin to digest and detach the glycoproteins from the plasma membrane. From the resulting glycopeptides, N-glycans were released and analyzed using MALDI-TOF mass spectrometry and HPLC. When N-glycan profiles obtained by this method were compared to those by other methods, the amount of high-mannose type glycans mainly contaminated from the endoplasmic reticulum was dramatically reduced, which enabled the efficient detection of complex type glycans present on the cell surface. Moreover, this method was successfully used to analyze the increase of high-mannose glycans on the surface as induced by a mannosidase inhibitor treatment.-
dc.publisherAmer Chem Soc-
dc.titleEfficient adhesion-based plasma membrane isolation for cell surface N-glycan analysis-
dc.title.alternativeEfficient adhesion-based plasma membrane isolation for cell surface N-glycan analysis-
dc.typeArticle-
dc.citation.titleAnalytical Chemistry-
dc.citation.number15-
dc.citation.endPage7470-
dc.citation.startPage7462-
dc.citation.volume85-
dc.contributor.affiliatedAuthorJi Young Mun-
dc.contributor.affiliatedAuthorHoon Seo-
dc.contributor.affiliatedAuthorMin-Sun Sung-
dc.contributor.affiliatedAuthorYee Sook Cho-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.alternativeName문지영-
dc.contributor.alternativeName이경진-
dc.contributor.alternativeName서훈-
dc.contributor.alternativeName성민선-
dc.contributor.alternativeName조이숙-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName오두병-
dc.identifier.bibliographicCitationAnalytical Chemistry, vol. 85, no. 15, pp. 7462-7470-
dc.identifier.doi10.1021/ac401431u-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Aging Convergence Research Center > 1. Journal Articles
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