Enhanced kinase sensing by radio-phosphorylation and oriented immobilization of a GBP-linked kinase substrate on a gold surface

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Title
Enhanced kinase sensing by radio-phosphorylation and oriented immobilization of a GBP-linked kinase substrate on a gold surface
Author(s)
J K Rho; J A Kang; M H Choi; Kyong-Cheol Ko; S H Park
Bibliographic Citation
Sensors and Actuators B-Chemical, vol. 160, pp. 632-636
Publication Year
2011
Abstract
A new strategy for enhanced sensing of kinases was developed using radio-phosphorylation and a polypeptide with an affinity for gold that was linked directly to kinase substrates. Gold-binding polypeptide (GBP)-linked GST-fusion substrates were efficiently immobilized on gold-coated slides, which is highly desirable for protein chips. The novel fusion substrates permitted oriented substrate immobilization on gold-coated slides, thereby enhancing the sensitivity of kinase assays. Thus, the proposed strategy is applicable to the development of protein microarrays and microfluidic lab-on-a-chip (LOC) sensing the activities of protein kinases.
Keyword
BiochipGold-binding peptideOriented immobilizationPhosphorylationRadioisotope detection
ISSN
0925-4005
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.snb.2011.08.040
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Korea Preclinical Evaluation Center > 1. Journal Articles
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