A transgene expression system for the marine microalgae Aurantiochytrium sp. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance

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dc.contributor.authorWon-Kyung Hong-
dc.contributor.authorSun-Yeon Heo-
dc.contributor.authorBaek Rock Oh-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorJ W Yang-
dc.contributor.authorA Kondo-
dc.contributor.authorJeong-Woo Seo-
dc.date.accessioned2017-04-19T09:41:56Z-
dc.date.available2017-04-19T09:41:56Z-
dc.date.issued2013-
dc.identifier.issn1615-7591-
dc.identifier.uri10.1007/s00449-012-0846-6ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11463-
dc.description.abstractIn the present study, we established a genetic system for manipulating the oleaginous heterotrophic microalgae Aurantiochytrium sp. KRS101, using cycloheximide resistance as the selectable marker. The gene encoding ribosomal protein L44 (RPL44) of Aurantiochytrium sp. KRS101 was first identified and characterized. Proline 56 was replaced with glutamine, affording cycloheximide resistance to strains encoding the mutant protein. This resistance served as a novel selection marker. The gene encoding the Δ12-fatty acid desaturase of Mortierella alpina, used as a reporter, was successfully introduced into chromosomal DNA of Aurantiochytrium sp. KRS101 via 18S rDNA-targeted homologous recombination. Enzymatic conversion of oleic acid (C18:1) to linoleic acid (C18:2) was detected in transformants but not in the wild-type strain.-
dc.publisherSpringer-
dc.titleA transgene expression system for the marine microalgae Aurantiochytrium sp. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance-
dc.title.alternativeA transgene expression system for the marine microalgae Aurantiochytrium sp. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance-
dc.typeArticle-
dc.citation.titleBioprocess and Biosystems Engineering-
dc.citation.number9-
dc.citation.endPage1197-
dc.citation.startPage1191-
dc.citation.volume36-
dc.contributor.affiliatedAuthorWon-Kyung Hong-
dc.contributor.affiliatedAuthorSun-Yeon Heo-
dc.contributor.affiliatedAuthorBaek Rock Oh-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorJeong-Woo Seo-
dc.contributor.alternativeName홍원경-
dc.contributor.alternativeName허선연-
dc.contributor.alternativeName오백록-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName양지원-
dc.contributor.alternativeNameKondo-
dc.contributor.alternativeName서정우-
dc.identifier.bibliographicCitationBioprocess and Biosystems Engineering, vol. 36, no. 9, pp. 1191-1197-
dc.identifier.doi10.1007/s00449-012-0846-6-
dc.subject.keywordAurantiochytrium-
dc.subject.keywordCycloheximide resistance-
dc.subject.keywordGene transformation-
dc.subject.keywordRibosomal protein-
dc.subject.localAurantiochytrium-
dc.subject.localCycloheximide resistance-
dc.subject.localgene transformation-
dc.subject.localGene transformation-
dc.subject.localRibosomal protein-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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