Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol

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Title
Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol
Author(s)
L L Luo; Jeong-Woo SeoSun-Yeon HeoBaek Rock Oh; D H Kim; Chul Ho Kim
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 36, no. 9, pp. 1319-1326
Publication Year
2013
Abstract
Klebsiella pneumoniae produces 3-hydroxypropionic acid (3-HP) from glycerol with oxidation of 3-hydroxypropionaldehyde (3-HPA) to 3-HP in a reaction catalyzed by aldehyde dehydrogenase (ALDH). In the present study, two putative ALDHs of K. pneumoniae, YneI and YdcW were identified and characterized. Recombinant YneI was specifically active on 3-HPA and preferred NAD+ as a cofactor, whereas YdcW exhibited broad substrate specificity and preferred NADP+ as a cofactor. Overexpression of ALDHs in the glycerol oxidative pathway-deficient mutant K. pneumoniae AK resulted in a significant increase in 3-HP production upon shake-flask culture. The final titers of 3-HP were 2.4 and 1.8 g L-1 by recombinants overexpressing YneI and YdcW, respectively. Deletion of the ALDH gene from K. pneumoniae did not affect the extent of 3-HP synthesis, implying non-specific activity of ALDHs on 3-HPA. The ALDHs might play major roles in detoxifying the aldehyde generated in glycerol metabolism.
Keyword
3-Hydroxypropionic acidAldehyde dehydrogenaseGlycerolKlebsiella pneumoniae
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-012-0880-4
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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