Characterization of a novel steviol-producing beta-glucosidase from Penicillum decumbens and optimal production of the steviol = Penicillum decumbens로부터 스테비올을 생산하는 글루코시다제의 특성 및 스테비올의 대량생산

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dc.contributor.authorJin A Ko-
dc.contributor.authorYoung Bae Ryu-
dc.contributor.authorHyung Jun Kwon-
dc.contributor.authorHyung Jae Jeong-
dc.contributor.authorSu-Jin Park-
dc.contributor.authorCha Young Kim-
dc.contributor.authorY J Wee-
dc.contributor.authorD Kim-
dc.contributor.authorWoo Song Lee-
dc.contributor.authorYoung-Min Kim-
dc.date.accessioned2017-04-19T09:41:58Z-
dc.date.available2017-04-19T09:41:58Z-
dc.date.issued2013-
dc.identifier.issn0175-7598-
dc.identifier.uri10.1007/s00253-013-4883-0ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11466-
dc.description.abstractThis study aimed to develop an economically viable enzyme for the optimal production of steviol (S) from stevioside (ST). Of 9 commercially available glycosidases tested, S-producing β-glucosidase (SPGase) was selected and purified 74-fold from Penicillium decumbens naringinase by a three-step column chromatography procedure. The 121-kDa protein was stable at pH 2.3-6.0 and at 40-60 C. Hydrolysis of ST by SPGase produced rubusoside (R), steviolbioside (SteB), steviol mono-glucoside (SMG), and S, as determined by HPLC, HPLC-MS, and 1H- and 13C-nuclear magnetic resonance. SPGase showed higher activity toward steviol mono-glucosyl ester, ST, R, and SMG than other β-linked glucobioses. The optimal conditions for S production (30 mM, 64 % yield) were 47 mM ST and 43 μl of SPGase at pH 4.0 and 55 C. This is the first report detailing the production of S from ST hydrolysis by a novel β-glucosidase, which may be useful for the pharmaceutical and agricultural areas.-
dc.publisherSpringer-
dc.titleCharacterization of a novel steviol-producing beta-glucosidase from Penicillum decumbens and optimal production of the steviol = Penicillum decumbens로부터 스테비올을 생산하는 글루코시다제의 특성 및 스테비올의 대량생산-
dc.title.alternativeCharacterization of a novel steviol-producing beta-glucosidase from Penicillum decumbens and optimal production of the steviol-
dc.typeArticle-
dc.citation.titleApplied Microbiology and Biotechnology-
dc.citation.number18-
dc.citation.endPage8161-
dc.citation.startPage8151-
dc.citation.volume97-
dc.contributor.affiliatedAuthorJin A Ko-
dc.contributor.affiliatedAuthorYoung Bae Ryu-
dc.contributor.affiliatedAuthorHyung Jun Kwon-
dc.contributor.affiliatedAuthorHyung Jae Jeong-
dc.contributor.affiliatedAuthorSu-Jin Park-
dc.contributor.affiliatedAuthorCha Young Kim-
dc.contributor.affiliatedAuthorWoo Song Lee-
dc.contributor.affiliatedAuthorYoung-Min Kim-
dc.contributor.alternativeName고진아-
dc.contributor.alternativeName류영배-
dc.contributor.alternativeName권형준-
dc.contributor.alternativeName정형재-
dc.contributor.alternativeName박수진-
dc.contributor.alternativeName김차영-
dc.contributor.alternativeName위영중-
dc.contributor.alternativeName김도만-
dc.contributor.alternativeName이우송-
dc.contributor.alternativeName김영민-
dc.identifier.bibliographicCitationApplied Microbiology and Biotechnology, vol. 97, no. 18, pp. 8151-8161-
dc.identifier.doi10.1007/s00253-013-4883-0-
dc.subject.keywordβ-Glucosidase-
dc.subject.keywordNaringinase-
dc.subject.keywordPenicillium decumbens-
dc.subject.keywordSteviol-
dc.subject.keywordStevioside-
dc.subject.localβ-Glucosidase-
dc.subject.localβ-glucosidase-
dc.subject.localΒ-glucosidase-
dc.subject.localNaringinase-
dc.subject.localPenicillium decumbens-
dc.subject.localSteviol-
dc.subject.localStevioside-
dc.subject.localstevioside-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
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