Comparison of the nucleotide sequence of cloned osteopontin from Hanwoo and Holstein

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Title
Comparison of the nucleotide sequence of cloned osteopontin from Hanwoo and Holstein
Author(s)
Tae Young Lee; S K Ju; M S Nam
Bibliographic Citation
Korean Journal for Food Science of Animal Resources, vol. 33, no. 5, pp. 331-334
Publication Year
2013
Abstract
Osteopontin (OPN) is a secreted phosphorylated glycoprotein. It has an important role in multiple biological processes including cell survival, bone remodeling, inhibition of ectopic calcification, as well as, is thought to have potential immune modulation activities. In this work, we isolated and characterized a full-length open reading frame (ORF) of Korean native cow's OPN from Korean native cow's (Hanwoo) kidney, and successfully cloned firstly on Hanwoo's OPN. The sequencing results indicated that the isolated cDNA was 1190 bp in length containing a complete ORF of 837 bp. It encoded a precursor protein Hanwoo's OPN consisting of 278 amino acids with a signal peptide of 16 amino acids. Amino acid homology was found to be 99.3% as compared to the corresponding sequences of Holstein bone marrow OPN. Hanwoo's kidney OPN and Holstein bone marrow OPN are different only in two amino acid residues 42 and 56, amino acid residue 42 is Thr (T) ↔ Ile (I), and amino acid residue 56 is Ala (A) ↔ Thr (T) respectively. These results from the present work would be helpful to elucidate the biological function of Hanwoo's OPN and provided a foundation for further insight into role of Hanwoo's OPN.
Keyword
Amino acidCloningKorean native cow (Hanwoo)Osteopontin
ISSN
1225-8563
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5851/kosfa.2013.33.3.331
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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