The UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine- phosphotransferase gene as a new selection marker for potato transformation
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Title
The UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine- phosphotransferase gene as a new selection marker for potato transformation
Author(s)
Hyun Soon Kim; M S Kim; K A Hwang; S W Park; Jae Heung Jeon
Bibliographic Citation
Bioscience Biotechnology and Biochemistry, vol. 77, no. 7, pp. 1589-1592
Publication Year
2013
Abstract
Here we describe the generation of potato plants that constitutively overexpressed, UDP-N-acetylglucosamine: dolichol phosphate-N-acetylglucosamine- phosphotransferase (GPT). Such transgenic plants can be formed in a medium with tunicamycin at 9:8 ± 0:28% efficiency, similar to the 9:4 ± 1:10 for the bialaphos resistance gene (Bar) gene. This study indicated that GPT transformation was very stable with high reproducibility, and that growth and tuber production in the GPT-transformed plants were stronger than in the wild-type plants.