Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii

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Title
Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii
Author(s)
Y E Choi; H Hwang; H S Kim; J W Ahn; Won Joong Jeong; J W Yang
Bibliographic Citation
Bioresource Technology, vol. 145, pp. 108-115
Publication Year
2013
Abstract
In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.
Keyword
Random mutagenesisChlamydomonas reinhardtiiComparative proteomicsMicroalgal lipid biosynthesis
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2013.03.142
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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