Regulation of transcription from two ssrS promoters in 6S RNA biogenesis

Cited 12 time in scopus
Metadata Downloads
Title
Regulation of transcription from two ssrS promoters in 6S RNA biogenesis
Author(s)
J Y Lee; H Park; G Bak; Kwang-sun Kim; Y Lee
Bibliographic Citation
Molecules and Cells, vol. 36, no. 3, pp. 227-234
Publication Year
2013
Abstract
ssrS-encoded 6S RNA is an abundant noncoding RNA that binds σ70-RNA polymerase and regulates expression at a subset of promoters in Escherichia coli. It is transcribed from two tandem promoters, ssrS P1 and ssrS P2. Regulation of transcription from two ssrS promoters in 6S RNA biogenesis was examined. Both P1 and P2 were growth phase-dependently regulated. Depletion of 6S RNA had no effect on growth-phase-dependent transcription from either promoter, whereas overexpression of 6S RNA increased P1 transcription and decreased P2 transcription, suggesting that transcription from P1 and P2 is subject to feedback activation and feedback inhibition, respectively. This feedback regulation disappeared in Δfis strains, supporting involvement of Fis in this process. The differential feedback regulation may provide a means for maintaining appropriate cellular concentrations of 6S RNA.
Keyword
6S RNAfeedback regulationFispromotersigma factor
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10059-013-0082-1
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.