A rapid detection of neopterin based on a label-free and homogeneous FRET immunoassay system

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Title
A rapid detection of neopterin based on a label-free and homogeneous FRET immunoassay system
Author(s)
T Li; Bobae Kim; W B Shim; J E Song; Yong Beom Shin; M G Kim
Bibliographic Citation
Proceedings of SPIE, vol. 8879, pp. 88790L-88790L
Publication Year
2013
Abstract
Herein, we have developed a label-free and homogeneous fluorescence resonance energy transfer (FRET) immunoassay for the detection of neopterin (NPT), which is an early and valuable biochemical marker of cellular immunity. Owing to intrinsic fluorescence properties of antibody and NPT, anti-NPT antibody (anti-NPT) and analyte played roles as the respective donor and acceptor in the FRET immunoassay. As the concentration of NPT increases, the fluorescence intensity at ~350 nm decreases owing to the formation of increasing amounts of the anti-NPT/NPT complex in which FRET takes place. The assay system was found to display a high specificity and a low detection limit (0.14 ng mL-1) for NPT. A practical application of the FRET immunoassay system was demonstrated by its use in the detection of NPT in spiked human serum samples. The observations made in these efforts show that the homogeneous FRET immunoassay strategy, which requires a simple sample preparation procedure, serves as a powerful tool for the rapid and sensitive quantitative determination of NPT.
Keyword
antibodybiomarkerFRET immunoassayHomogeneousLabel-freeNeopterin
ISSN
0277-786X
Publisher
International Society for Optical Engineering
DOI
http://dx.doi.org/10.1117/12.2018671
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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